摘要
目的:探讨乙型肝炎病毒(HBV)慢性感染者外周血IL-10、IFN-γ及IL-2的表达水平及其临床意义。方法:分离63例HBV感染者血清(无症状携带者组21例,慢性乙肝患者轻度组和中、重度组各21例),双抗体夹心法ELISA定量检测血清的IL-10、IFN-γ及IL-2表达水平,10例健康献血者为对照组。结果:中、重度组患者IFN-γ水平较对照组、无症状携带者组和轻度组明显升高(P<0.05);其中各组HBV-DNA高载量者(≥1.0×105)IL-10、IFN-γ水平均较低载量者(<1.0×105≥1.0×103)和对照组明显升高(P<0.05);轻度和中、重度组的IL-10水平明显高于对照组(P<0.05),无症状携带者及中、重度组IL-10水平明显高于轻度组(P<0.05);各组IL-2水平无显著差异(P>0.05)。结论:乙型肝炎病毒慢性感染者免疫应答异常与IL-10及IFN-γ表达水平有关;携带者IL-10浓度升高可能是诱导的免疫耐受的因素之一。
Objective：To investigate the significance of serum IL-10、IL-2 and IFN-γ level changes in Hepatitis B Virus infection patients.Methods：The serum IL-2、IFN-γ and IL-10 level of 63 patients who had infected with Hepatitis B,including 21 HBV carriers and 42 chronic HBV patients（21 patients with mild degree,21 patients with moderate or serious degree）were detected by ELISA.10 healthy human were detected as control group.Results：The serum IFN-γ level in patients with moderate or serious degree group were obviously higher than the HBV carriers group、mild degree group and control group（P〈0.05）.The serum IL-10 and IFN-γ level in patients with high hepatitis B virus load was obviously higher than control group and low virus load group（P〈0.05）.The serum IL-10 level in patients was obviously higher than control group（P〈0.05）.Both the HBV carriers group and patients with moderate or serious degree group were obviously higher than mild degree group（P〈0.01）.Conclusion：The serum IFN-γ and IL-10 level may induce the disbalance immune state of the patients with persistent HBV infection.The higher serum IL-10 level in HBV carriers may induce immune tolerance.
出处
《长治医学院学报》
2008年第3期175-177,共3页
Journal of Changzhi Medical College
基金
山西省自然科学基金(2008011073-4)
