摘要
目的:分析急性肺损伤中IL-6与肺表面活性蛋白-A(surfactant protein A,SP-A)和SP-B的含量变化及其相互关系,探讨急性肺损伤的发病机制。方法:用Wistar大鼠复制油酸性急性肺损伤动物模型,在不同时间点收集急性肺损伤大鼠血清标本及肺泡灌洗液(bronchoalveo larlavage fluid,BALF),用放射免疫法测定血清细胞因子IL-6的动态变化;采用ELISA法检测BALF中SP-A、SP-B含量的变化。结果与结论:急性肺损伤后大鼠血清中细胞因子IL-6的分泌水平显著上升(P<0.05),而BALF中SP-A、SP-B含量显著降低,且血清中IL-6含量升高与BALF中SP-A、SP-B下降幅度呈显著负相关。结果提示急性肺损伤中IL-6分泌的升高与肺表面活性蛋白SP-A、SP-B含量的降低有显著的相关关系,炎症细胞因子IL-6可能通过影响SP-A、SP-B的表达产生致病作用。
Objective：To investigate the relationship between IL-6 and surfactant proteins A and B in the pathogenesis of acute lung injury （ALI）. Methods： ALI modes were established using Wistar rats. Serum and bronchoalveolar lavage fluid （BALF） samples were collected from ALI models at different time points. The level of IL-6 in the serum was determined using radio immunoassay. The concentration of SP-A and SP-B in the BALF was analyzed by ELISA, The relationship between IL-6 and SP-A and SP-B in the process of ALI was analyzed, Results ： IL-6 levels in the serum were significantly upregulated（ P 〈0.05 ）,while the concentrations of SP-A and SP-B significantly decreased after ALI（ P 〈 0.05 ）, The data showed a negative correlation between IL-6 and SP-A, SP-B in the pathogenesis of ALI. Conclusion ： The results suggested that the up-regulated pro-inflammatory cytokine IL-6 may exacerbate the process of ALI act by damaging the production of SP-A and SP-B.
出处
《军事医学科学院院刊》
CSCD
北大核心
2008年第3期261-263,268,共4页
Bulletin of the Academy of Military Medical Sciences
