摘要
目的探讨纳米脂质体承载榄香烯诱导C6胶质瘤细胞凋亡的作用以及对相关基因caspase-3表达的影响。方法以同等剂量的榄香烯-纳米脂质体、普通榄香烯和空白培养液作用于C6胶质瘤细胞,分别于0、48、72h时通过流式细胞仪检测C6胶质瘤细胞的凋亡情况,以免疫组织化学法检测Caspase-3蛋白的表达。结果榄香烯-纳米脂质体和普通榄香烯组细胞瘤于48h和72h时出现了明显的凋亡,而且其凋亡率明显高于空白对照组。榄香烯-纳米脂质体和普通榄香烯组细胞瘤48h和72h时出现了明显的Caspase-3蛋白表达,而且其蛋白表达率也明显高于空白对照组。结论榄香烯具有促进C6胶质瘤细胞凋亡和Caspase-3蛋白表达的作用,经纳米脂质体承载后榄香烯的这种作用有增强的趋势。
Objective To study the effect of nano-liposome sustained elemene in inducing cell apoptosis of C6 glioma and to explore its influence on the expression of caspase-3 gene. Methods C6 glioma cells were cultured in medium with the same amount of nano-liposome sustained elemene and common elemene respectively, also in blank medium for control. The status of cell apoptosis was determined by flow cytometry at 0, 48 h and 72 h, and the ex- pression of Caspase-3 protein was measured simultaneously by immunohistochemistry assay. Results Marked apoptosis presented in cells cultured in the medium with nano-liposome sustained elemene or common elemene at 48 h and 72 h, with the apoptotic rate significantly higher than that in the control. At the same time, Caspase-3 protein expression raised significantly in cells cultured in medium with either kinds of elemene, showing significant difference when compared with that in the control. Conclusion Elemene has significant apoptosis promoting and Caspase-3 protein expression inducing effect on C6 glioma cells, which could be facilitated by nano-liposome bearing.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第7期637-639,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省中医药管理局(No2007CA060)
