摘要
目的:研究风湿之邪对不同温度梯度变化的寒邪犯肺证大鼠肺脏组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)、白细胞介素4(interleukin-4,IL-4)、γ干扰素(interferon-γ,IFN-γ)等细胞因子的影响。方法:将104只SPF级Wistar大鼠随机分为13组:常温对照组、寒邪6组和风寒湿邪(寒邪夹5级左右风力,相对湿度为90%～100%)6组,寒邪包括恒定低温(10℃、0℃、-10℃)和温差(20～10℃、20～0℃、20～-10℃)。第1天将大鼠置于相应环境的调温箱中饲养2h后取出,置常温下饲养4d,观察大鼠的整体状况。放射免疫法测肺组织匀浆中TNF-α、IL-6和IL-4含量,酶联免疫吸附试验检测肺组织匀浆中IFN-γ含量。结果:与相同温度的寒邪组相比,风寒湿各组大鼠肺组织匀浆中TNF-α、IL-6和IL-4水平明显升高(P<0.01),IFN-γ水平显著下降(P<0.01),IFN-γ/IL-4比值显著下降(P<0.01)。结论:风湿之邪能够明显加重寒邪对肺脏的损害,加重寒邪犯肺所导致的机体Th1/Th2免疫应答失衡。
Objective： To explore the effects of wind and dampness pathogens on cytokines in the lung tissue of rats with cold syndrome due to different gradient cold pathogens.
Methods： One hundred and four Wistar rats of SPF grade were randomly divided into 13 groups： normal temperature group, six cold pathogen groups and six cold plus wind-dampness pathogen （wind of grade 5 and 90%-100% relative humidity） groups. The cold pathogens were constant low temperature （including 10 ℃, 0 ℃. --10℃） and temperature change （including 20 to 10 ℃, 20 to 0 ℃, and 20 to--10 ℃）. The rats in different groups were kept in a temperature-controlled box under the corresponding condition for 2 hours on the first day of experiment. Then, the rats were all raised in normal temperature for 4 days and the rats behaviors were observed. The contents of tumor necrosis factor α （TNF-α）, interleukin-6（IL-6） and interleukin-4（IL-4） in lung homogenate were measured by radioimmunoassay and the content of interferon-γ （IFN-γ） was detected by enzyme-linked immunosorbent assay.
Results： In comparison with cold pathogen groups, contents of TNF-α, IL-6 and IL-4 were obviously increased in lung homogenate of rats in cold plus wind-dampness pathogen groups （ P 〈0.01）, and the content of IFN-γ and IFN-γ/IL-4 ratio were obviously decreased （ P 〈0.01）.
Conclusion： Wind-dampness pathogen can seriously aggravate the injury to lung tissue caused by cold pathogen, and the unbalance of Th1/Th2 in lung homogenate of rats.
出处
《中西医结合学报》
CAS
2008年第7期748-751,共4页
Journal of Chinese Integrative Medicine
基金
山东省卫生系统高层次人才1020工程资助课题
山东省卫生厅计划课题(No2005-215)
关键词
寒邪
风邪
湿邪
大鼠
寒邪客肺
细胞因子
pathogenic cold
pathogenic wind
pathogenic dampness
rats
pathogen-cold invading lung
cytokines
