摘要
为在ＳＣＩＤ小鼠体内获得人免疫Ｂ淋巴细胞，将１２～１４周龄的胎儿肝细胞（１～２）×１０１０／Ｌ腹腔注射于ＳＣＩＤ小鼠，然后再分次接种肝癌细胞。用ＥＬＩＳＡ法于第４，５，６周连续检测ＳＣＩＤ－ｈｕ小鼠血清人ＩｇＧ；用免疫组化ＡＢＣ法检测各种组织中的人淋巴细胞。各实验小鼠均检测到人ＩｇＧ的存在，最高为３９１μｇ／Ｌ，且随时间的延长呈上升趋势。在ＳＣＩＤ－ｈｕ小鼠肝、脾及腹腔接种瘤组织的周边，可见人ＣＤ３＋和ＣＤ２０＋的淋巴细胞。上述结果表明，用人胎肝细胞移植可在ＳＣＩＤ小鼠体内获得人淋巴细胞，并对人肝癌细胞抗原产生免疫应答，分泌一定水平的抗人肝癌的抗体。提示：用人胎肝细胞在ＳＣＩＤ小鼠体内可建立人免疫系统的部分功能。
Immunodifecient C.B17 scid/scid(SCID) mice were reconstituted with fetal liver cells and immunized with human hepatic carcinomatous cells (HHCC). All of the adaptively transfered mice had serum levers of 64391 μg/L of human IgG 4￣6 weeks after reconstitution, and showed characteristic response to HHCC. The human CD3+, CD20+ lymphocytes were found residing in the liver, spleen and vicinity of the injected tumor tissue. These results suggest that SCID mice, repopulated with fetal cells, can constitute a functional model and provide a source for human immune lymphocytes.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1997年第4期41-44,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金
