摘要
目的:研究转染HBV的HepG2.2.15细胞株在体外促进肝星状细胞中Ⅰ、Ⅲ型胶原的表达,进而探讨HBV促肝细胞纤维化的机制.方法:将HepG2和HepG2.2.15细胞株分别在体外与肝星状细胞共培养,以单独培养的肝星状细胞为对照组.取培养后24、48、72h3个时间点,以RT-PCR定量检测肝星状细胞中Ⅰ、Ⅲ型胶原mRNA的表达;以Western blot定量检测肝星状细胞中Ⅰ、Ⅲ型胶原蛋白的表达.结果:与对照组和与HepG2共培养的肝星状细胞比较,与HepG2.2.15共培养的肝星状细胞中Ⅰ、Ⅲ型胶原mRNA的表达明显增高,以72h差异最为显著(P<0.01);与HepG2.2.15共培养的肝星状细胞中Ⅰ、Ⅲ型胶原蛋白的表达也明显增高,以48h差异最为显著(P<0.01).结论:与HepG2.2.15细胞株共培养后,肝星状细胞中肝纤维化相关因子的表达明显增强,HBV具有诱导肝细胞纤维化的重要作用.
AIM： To investigate whether HepG2.2.15 could induce expression of fibrosis-related factors in hepatic stellate cells in vitro and further to explore the mechanism of HBV inducing fibrogenesis. METHODS： The hepatic stellate cells were co-cultured with HepG2 or HepG2.2.15 in vitro and the hepatic stellate cells cultured alone were used as control. For differentiation mRNA expression of Collagen Ⅰ and Ⅲ in hepatic stellate cells, real-time PCR was performed; for differentiation protein expression of Collagen Ⅰ and Ⅲ inhepatic stellate cells, Western blot analysis was performed. RESULTS： Compared with the control and the hepatic stellate cells co-cultured with HepG2, mRNA expression of Collagen Ⅰ and Ⅲ were significantly higher in the hepatic stellate cells co-cultured with HepG2.2.15 and the most prominent effect was found at 72 h （P 〈 0.01）; the protein expression of Collagen Ⅰ and Ⅲ were higher signifi cantly in the hepatic stellate cells co-cultured with HepG2.2.15 and the most prominent effect was found at 48 h compared with control and the hepatic stellate cells co-cultured with HepG2 （P 〈 0.01）. CONCLUSION： The expression of fibrosis-relat-ed factors in hepatic stellate cells are increased greatly after being co-cultured with HepG2.2.15. HBV is capable of inducing fibrogenesis in vitro.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第18期2031-2035,共5页
World Chinese Journal of Digestology
基金
国家十五科技攻关计划资助项目
No.2004BA718B10~~
