摘要
以幼嫩叶片为材料,经榨汁机破碎组织和细胞,采用差速离心法分离出线粒体,然后经Sarkosyl和CTAB裂解沉淀、氯仿/异戊醇反复抽提、RNA酶解等步骤分离纯化m tDNA。检测结果表明,采用该方法提取的m tDNA纯度高、污染少、电泳条带清晰。
Take leaflet as materials. Tissues and cells has been mitochondrion was isolated by different speed centrifugation and lysed broken with juicers. And the with Sarkosyl and CTAB. The lysate was extracted with phenol/chloroform and digested with RNAase. By examination with agrose - gel electrophoresis and UV spectrophotometry, mtDNA with this method was high quality and not destroyed.
出处
《林业科技》
北大核心
2008年第4期57-59,共3页
Forestry Science & Technology
基金
宁夏自然科学基金(NZ20690)资助
关键词
枸杞
线粒体DNA
Lycium barbarum
Mitohondrial DNA
