摘要
采用反相高效液相色谱法（RP-HQLC）测定了罗布麻叶中槲皮素和山萘酚。色谱柱为Nova-pak C18（15cm×4．6mm i.d.），流动相为A．甲醇、B．1％乙酸，流速为0．6mL／min，检测波长为360nm。槲皮素在0．0137～0．136μg、山萘酚在0．0053～0．0265μg范围内与峰面积呈良好的线性关系，相关系数分别为0．9998和0．9980。槲皮素的回收率为99．6％，RSD为0．97％；山萘酚的回收率为98．2％，RSD为2．0％。方法可用于罗布麻叶中槲皮素和山萘酚的测定。
A method for determining quercetin and kaempferol in Apocynum venetum L. by RP-HPLC was described. he determination was carried out on a Nova-pak C,s column （15 cm×4.6 mm） at room temperature. 1% CH3COOH and MeOH was used as the mobile phase at a flow rate of 0.6 mL/min and detection wavelength was set at 370 nm. The calibration curves were linear in the concentration range of 0.0137 - 0.136 μg for quercetin and 0.0053 - 0.0265 μg for kaempferol. Correlation coefficients of quereetin and kaempferol were 0. 9998 and 0. 9980 respectively. The recoveries of quercetin and kaempfero were 99.6 % （RSD=0.97%,n=3） and 98.2 % （RSD=2.04%, n =3） respectively. This method is simple, specific and accurate for monitoring concentrations of quercetin and kaempferol in Apocynum venetum L..
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第8期52-54,共3页
Chinese Journal of Analysis Laboratory
关键词
罗布麻叶
槲皮素
山萘酚
反相高效液相色谱法
Apocynum venetum L.
Quercetin
Kaempferol
Reversed-phase high performanceliquid chromatography
