摘要
目的：探讨雷诺昔芬对骨质疏松大鼠实验性牙周炎牙槽骨吸收的防治作用。方法：选用3-4月龄雌性SD大鼠,随机分为假手术组（SHAM）、卵巢切除组（OVX）、雷诺昔芬治疗组（RAL）及雌激素治疗组（EST）,每组8只。采用卵巢切除术及钢丝结扎法建立骨质疏松大鼠实验性牙周炎动物模型,灌胃给药7周后处死,通过体重、骨密度测量、血清生化检测及组织学观察对药效进行评价。结果：RAL组大鼠骨密度值明显高于OVX组,血清碱性磷酸酶及骨钙素水平下降,而血清雌二醇水平显著增加。股骨骨小梁排列整齐,皮质骨致密,与EST组及SHAM组比较无明显差别。OVX组牙周炎牙槽骨吸收（0.33±0.02）mm2,明显大于RAL、EST组及SHAM组（0.18±0.02）mm2、（0.19±0.02）mm2、（0.20±0.01）mm2（P〈0.01）。结论：雷诺昔芬可在一定程度上抑制骨质疏松大鼠实验性牙周炎所致病理性牙槽骨吸收。
Objective：To evaluate whether raloxifene can affect periodontitis-associated bone loss in osteoporotic rats. Methods：Thirty two three to four months old female SD rats were randomly divided into four groups as following： sham operated, ovariectomized（OVX）, ovariectomized plus estrogen administration （EST） and ovarieetomized plus raloxifene administration（RAL）. After one week, steel-wire was placed around one of the lower first molar of all rats to induce periodontal diseases. Seven weeks later, rats were sacrificed and the specimens routinely processed. Body weight, bone mineral density（BMD） , bone histomorphometry and serum biochemical indices were investigated. Results：The body weight of the OVX rats were significantly increased （P 〈 0.01 ）. But B MD of total body, lumbar spine and femur in OVX rats were lower than those in the rats treated with raloxifene and estrogen. Compared with the OVX, the alkaline phosphatase and bone r-carboxyglutamic acid protein in serum of RAL and EST were obviously decreased while the estradiol was increased（ P 〈0.05 ）. Histomorphometry indicated the raloxifene and estrogen can prevented ovariectonlized induced bone loss. Intergroup analysis revealed that an estrogen-deficient state significantly increased bone loss resulting fronl periodontitis and could be prevented by estrogen and raloxifene administration （0.33 ± 0.02, 0.18 ± 0.02, 0.19 ± 0.02, 0.20 ± 0.01 for OVX, RAL, EST and SHAM） （ P 〈 0.01 ）. Conclusion ： Raloxifene administration can prevent bone loss to reduce osteoporosis in ovariectomized rats and prohibit periodontitis-associated alveolar bone resorption.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2008年第4期491-495,共5页
Journal of Practical Stomatology
基金
福建省教育厅项目(JA07104)
福建医科大学教授学术发展基金项目(JS06104)
