摘要
目的:用快速分离液相色谱法分离测定人参中人参皂苷 Rg_1、人参皂苷 Re 和人参皂苷 Rb_1的含量。方法:采用 ZOBAX SB-C_(18)柱(1.8 μm,3.0 mm×50 mm);流动相:乙腈(A)-水(B),梯度洗脱(0～14 min,19%A;14～24 min,19%A→36%A;24～26min,36%A);流速:1.0 mL·min^(-1);检测波长:203 nm;柱温:35℃。结果:人参皂苷 Rg_1、人参皂苷 Re 和人参皂苷 Rb_1的线性范围分别为0.077～1.537 μg、0.058～1.156 μg和0.078～1.563 μg,相关系数均为0.9999。平均加样回收率(n=6)分别为98.3%,98.7%,99.2%;RSD 分别为0.9%,1.0%,0.5%。结论:本方法具有快速、准确,重复性好等特点,适合于人参的含量测定。
Objective：To establish a method to determine the contents of ginsengnoside Rg1, ginsengnoside Re and ginsengnoside Rb1 in radix et rhizoma Ginseng by rapid resolution liquid chromatography （RRLC）. Method：ZOBAX SB- C18 column（1.8 μm,3. 0 mm ×50 mm）;The mobile phase consisted of acetonitrile（A） -water（ B）with gradient elution （0 - 14 min, 19% A; 14 - 24 min, 19% A→36% A ;24 - 26 rain,36% A） at a flow rate of 1.0mL · min^-1 ; the wavelength of detector was 203 nm. Results： The calibration curves showed good linearity in the range of 0. 077 - 1. 537 μg（ginsenoside Rg1 ） ,0. 058 - 1. 156 μg（ginsenoside Re） and 0. 078 - 1. 563 μg（ginsenoside Rb1 ）. The average recoveries（ n = 6） were 98.3% ,98.7% and 99.2% , RSD were 0. 9% , 1.0% and 0. 5%. Conclusion： This method is high - speed, accurate and reproducible, and can assess the quality of ginseng.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第7期1144-1146,共3页
Chinese Journal of Pharmaceutical Analysis
