摘要
目的测定益母草不同药用部位中盐酸水苏碱的含量,以控制药材质量。方法采用HPLC法,色谱柱为磺酸基键合硅胶,流动相为20mmol/L磷酸二氢钠溶液(含有0.04%三乙胺和0.15%磷酸),柱温25℃;检测波长192nm,比较益母草花、茎、叶中盐酸水苏碱的含量。结果益母草中盐酸水苏碱的含量叶中最高,茎中最少。结论购买益母草药材以叶(包括碎叶片)多者为佳。
Objective To control the quality of Herba Leonuri by studying the content of stachydrine hydrochloride in different parts of it. Method A HPLC method was developed for the content detetmination of stachydrine hydrochloride in flower, stem, leafage from Herba Leonuri. Spherisorb SCX colum was used with mobile phase of 20 mmol/L sodium dihydrogen phosphate （containing 0.04% triethylamine and 0.15% phosphoric acid）. The colum temperature was 25 ℃, the detective wavelength was 192 nm. Results Stachydrine hydrochloride content was the highest in leafage and the lowest in stem. Conclusion To ensure the quality of Herba Leonuri, it is significant to choose medical material with more leafage.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第8期51-52,共2页
Chinese Journal of Information on Traditional Chinese Medicine
