摘要
采用PCR技术对河南郑州禾谷孢囊线虫群体的核糖体基因(ribosomal DNA,rDNA)内转录间隔区(Internal Tran-scribed Spacers,ITS)进行扩增,获得片段长度约为1 040 bp。利用UPGMA方法分析了河南郑州禾谷孢囊线虫群体与近缘种的系统发育关系,结果表明:中国Heterodera avenae群体,H.australis和H.pratensis亲缘关系很近。8种限制性内切酶(Restriction Enzyme,RE)酶切禾谷孢囊线虫ITS的扩增产物,其中HindⅢ、AvaⅠ不能酶切PCR产物;AluⅠ酶切PCR产物,获得560 bp和480 bp 2个片段;RsaⅠ和HinfⅠ酶切后分别得到3个片段(700、320、20 bp和820、180、40 bp);CfoⅠ是3个酶切位点(740、150、110、40 bp);HaeⅢ和MvaⅠ能分别清晰地观察到3个片段(420、350、180 bp和400、340、280 bp),但有微小片段无法清晰观察到。9个种群所得RFLP图谱一致,说明郑州禾谷孢囊线虫群体可能是同一种群且不同于欧洲群体(type A)和印度群体(type B)的C型。
The amplification of the rDNA-ITS region of Heterodera avenae （CCN） populations collected from Zhengzhou, Henan province with the current primers AB28 and TW81 yielded one fragment of approximately 1 040 bp. The result of ITS sequences analysis and alignments between H. avenae populations from Zhengzhou and related species H. australis and H. pratensis using UPGMA showed that it was clustered in a same group with close relationship. A total 23 scored fragments were obtained with 8 restriction enzymes. Hind Ⅱ and Ava Ⅰ couldn＇ t digest the ITS products of nine CCN populations from Zhengzhou. Alu Ⅰ digested PCR amplified ITS products of 9 populations obtained 2 fragments （560,480 bp）. Hinf Ⅰ and Rsa Ⅰ digested ITS products of 9 populations yielded 3 fragments respectively （820, 180, 40 bp and 700,320, 20 bp）. Digestion by Cfo Ⅰ obtained 4 fragments （740, 150, 110, 40 bp）. Digestion by HaeⅢ and Mva Ⅰ could observe 3 fragments （420, 350, 180 bp and 400, 340, 280 bp） but not small fragments. 8 restriction enzymes produced restriction profiles identical for all CCN populations. The results showed that 9 populations might be type C of H. avenae and different from type A of European populations and type B of Indian populations.
出处
《植物病理学报》
CAS
CSCD
北大核心
2008年第4期407-413,共7页
Acta Phytopathologica Sinica
基金
"十一五"国家科技支撑计划2006BAD08A15
国家自然科学基金资助项目(39970487)
中国农科院与意大利国家研究委员会2008~2010年科技合作项目
