摘要
为了探索血小板冰冻保存后膜表面促凝血活性相关分子变化与冰冻血小板体内即刻止凝血功能增强之间的可能联系,用流式细胞术检测了新鲜血小板冰冻保存前后Ⅴ因子结合能力、血小板膜表面GPIbⅨ--Ⅴ分子(CD42a)密度,用血凝仪测定激活血小板诱导血浆凝固时间(aPACT)变化,用血细胞计数仪测定血小板计数、MPⅤ和PDW。研究结果表明,与新鲜血小板比较,深低温保存后血小板激活血小板诱导血浆凝固时间缩短43.9%;结合Ⅴ因子的荧光强度平均增加117%;结合膜表面GPIbⅨ--Ⅴ分子的荧光强度增加32%。结论:冰冻血小板体内即可止凝血功能增强,可能与血小板冰冻保存后膜表面促凝血活性分子表达增加或功能增强,促凝血功能明显增强,发挥快速止血功能有关。
The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor V, density of GPIb-Ⅸ-V （CD2a） at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MP V and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved. The results showed that the clotting time induced by activated cryopreserved platelets decreased by 43.9 %, even quicker than that induced by fresh platelets; the fluorescence intensity of cryopreserved platelet binding factor V increased by 117%, more than that of fresh platelets binding factor V; the GPIb-Ⅸ-V （CD2a） density at cryopreserved platelet membrance surface increased by 32%, higher than that at fresh platelet surface. It is concluded that the enhancement of instant hemostatic function in vivo of cryopreserved platelet may be related to higher expression of procoagulative molecules or to their enhanced activity and rapid hemostatic effect.
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第4期930-932,共3页
Journal of Experimental Hematology
基金
军队医药卫生"十一五"重点课题
编号06Z59
关键词
血液保存
深低温保存
血小板
促凝血活性
blood preservation
cryopreservation
platelet
procoagulative activities
