摘要
目的研究分析临床感染患者分离的肠球菌属细菌耐药性及耐药基因。方法对临床分离出的72株肠球菌属进行菌株鉴定、药敏试验和氨基糖苷类〔aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(6)-Ⅰ〕、大环内酯类(ermB)、四环素类(tetM)、糖肽类耐药相关基因(vanA、vanB)和Ⅰ类整合子(IntⅠ)检测。结果72株肠球菌属对抗菌药物耐药率分别为青霉素(41.67%)、高浓度庆大霉素(86.11%)、高浓度链霉素(88.88%)、红霉素(83.33%)、四环素(52.78%)、呋喃妥因(16.67%)、左氧氟沙星(27.78%)、万古霉素(0);72株肠球菌属共检出aac(6′)/aph(2″)基因60株,aph(3′)-Ⅲ基因44株,ant(6)-Ⅰ基因56株,ermB基因52株,tetM基因38株,IntⅠ基因32株,未检出vanA和vanB基因。结论肠球菌属对临床常用的抗菌药物皆不同程度存在耐药,尚未发现耐万古霉素肠球菌,临床医生应重视肠球菌属所致感染的诊断与治疗,合理应用抗菌药物,避免耐药菌株造成的医院感染暴发流行。
OBJECTIVE To study the drug resistance and drug-resistant genes of Enterococcus from clinical infective patients. METHODS The drug resistance aac（6＇）/aph（2＂）, aph（3＇）-Ⅲ and ant（6）-I genes of aminoglycoside, ermB gene of macrolide, tetM gene of tetracycline, vanA and vanB genes of glycopeptides and Int I were detected in 72 Enterococcus strains. RESULTS In all 72 Enterococcus strains, 60 isolates were with positive aac（6＇）/aph （2＂）, 44 isolates were with positive aph（3＇）-Ⅲ , 56 isolates were with positive ant（6）-I , 52 isolates were with positive ermB, 38 isolates were with positive tetM, 32 isolates were with positive Int I , and none Enterococcus isolates were with positive vanA or vanB. CONCLUSIONS Enterococcus are the conditioned pathogens. In recent years, because of antibiotic extensive application, the Enterococcus infection is increasing continuously, having become one of the major pathogens responsible for hospital-acquired infection. Enterococcus are resistant to clinically common used 7 kinds of antibiotics in different degree excluding vancomycin. The clinical doctor should value the diagnosis and treatment of Enterococcus and adopt the different treatment projects to meet the different characteristics of Enterococcus＇infection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第8期1057-1060,共4页
Chinese Journal of Nosocomiology
关键词
肠球菌属
耐药基因
耐药性
整合子
Enterococcus
Drug-resistant gene
Drug resistance
Integron
