期刊文献+

蛋白质组技术在昆虫抗药性机理研究中的应用

Application of the Proteomic Technique in the Study on the Insecticide Resistance Mechanism
在线阅读 下载PDF
导出
摘要 主要介绍了蛋白质组学研究技术的主要途径及不同途径存在的问题、研究意义和利用该技术的研究成果,以及采用蛋白质组学技术研究杀虫剂作用下昆虫体内差异表达蛋白质的情况及意义,最后对该项技术的应用前景作了展望。 The prevalent technique route of the proteome research and drawback of different route,as well as its significance and main achievements were introduced.This technique was used to explore protein differential expression in response to pesticide,and its significance and research status were referred.Finally,we made a prospective of the application of this technique.
出处 《安徽农业科学》 CAS 北大核心 2008年第21期9169-9170,共2页 Journal of Anhui Agricultural Sciences
基金 安徽省教育厅自然科学重点基金项目(kj2008A137)
关键词 蛋白质组学 昆虫 差异表达 Proteome Insect Differential expression
  • 相关文献

参考文献23

  • 1WILKINS M.Government backs proteome proposal[J] .Nature ,1995,378:653.
  • 2JURI R,MATTHIAS M.What does it meanto identify a proteinin proteomics ?[J] .Trends in Biochemical Sciences ,2002 ,27(2) :74.
  • 3CHEVALLET M,SANTONI V,POINAS A,et al .Newzwitterionic detergents improve theanallysis of membrane proteins bytwo-dimensional electrophoresis[J] .Electrophoresis ,1998 ,19:1901 - 1909 .
  • 4ROEPSTORFF P.Mass spectrometry in protein studies fromgenome to function [J] .Curr Opinin Biotechnol ,1997 ,8 :6- 13.
  • 5GRIFFINTJ,GOODLETT D R,AEBERSOLD R.Advances in proteome analysis bymass spectrometry[J] .Curr Opin Biotechnol ,2001 ,12(6) :607 .
  • 6MANNM,HENDRICKSONRC,PANDEY A.Analysis of proteins and proteomes by mass spectrometry[J] .Annu Rev Biochem,2001 ,70 :437 .
  • 7PANDEY A,MANN M.Proteomics to study genes and genomes[J] .Nature ,2000 ,405:837.
  • 8CORBETTJ M,DUNNMJ ,POSCHA,et al .Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilized pHgradient isoelectric focusing in the first dimension:Aninterlaboratory comparison[J] .Electrophoresis ,1994 ,15:1205 .
  • 9FERMANDEZ J,GHARAHDAGHI F,MISCHE S M.Routine identification of proteins from sodiumdodecyl sulfate polyacrylamide gel electrophoresis( SDSPAGE)gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization-time of flight-mass spectrometry( MALDI-TOF-MS) [ J] .Electrophoresis ,1998 ,19:1036 .
  • 10CHENP,XIEJ Y,LIANGS P.Identification of proteinspots insiliver-stained two-dimensional gels by MALDI- TOF mass peptide map analysis [J] .Acta Biochim Biophys Sin,2000 ,2:387.

二级参考文献40

共引文献74

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部