摘要
目的研究 N-乙酰半胱氨酸(NAC)对镉致大鼠肝线粒体损伤的影响。方法取6只清洁级雄性 Wistar 大鼠肝脏,梯度离心得线粒体液。将所得线粒体液分为6组,分别为对照组、镉染毒组(CdCl_2的浓度分别为10、100、1000、10000μmol/L)和 NAC 预处理组(线粒体液先用500 μmol/L 的 NAC 预处理30min 后,加1000μmol/L 的 CdCl_2)。于培养箱内37℃孵育1 h 后,测定各组锰-超氧化物歧化酶(Mn-SOD)活力和还原型谷胱甘肽(GSH)、细胞色素 c(Cyt c)含量。结果与对照组相比,100、1000、10000μmol/L 镉染毒组 GSH 含量和 Mn-SOD 活力较低,差异均有统计学意义(P<0.05),且在10～10000μmol/L 范围内,随着 CdCl_2剂量的增加而呈降低趋势;1000、10000 μmol/L 镉染毒组 Cyt c 含量较高,差异均有统计学意义(P<0.05),且在0～10000μmol/L 范围内,随着 CdCl_2剂量的增加而呈升高趋势。与 NAC 预处理组相比,0、10、100、1000、10000 μmol/L 镉染毒组 GSH 含量和 Mn-SOD 活力以及0 μmol/L 镉染毒组 Cyt c 含量较低,1000、10000μmol/L 镉染毒组 Cyt c 含量较高,差异均有统计学意义(P<0.05)。结论镉可剂量依赖性地诱导大鼠肝线粒体氧化损伤,NAC 能有效预防镉所致大鼠肝线粒体氧化损伤。
Objective To study the antagonistic effect of N-acetylcysteine （NAC） on the damage of hepatic mitochondria of rats induced by cadmium in vitro. Methods The mitochondria were prepared from the clean Wistar rats＇ whole liver by using differ ential centrifugation. The mitochondria were incubated in the assay buffer containing different concentration of CdCl2 （10,100,1000,10 000 umol/L） at 37 ℃ for 1 h. The effect of NAC （500 umol/L） was studied at a CdCl2 concentration of 1 000 umol/L. The incubation buffer was collected and the level of GSH,cytochrome C and the activity of Mn-SOD were determined. Results Compared with the control group,the level of GSH and Mn-SOD in 100,1 000,10 000 umol/L CdCl2 groups were significantly decreased, the content of cytochrome C in 1 000,10 000 umol/L CdCl2 groups were significantly increased （P〈0.05）. In 500 umol/L NAC pretreated group, the level of GSH and the activity of Mn-SOD was significantly increased and the content of cytochrome C was significantly decreased compared with the 1 000 umol/L CdCl2 group （P〈0.05）. Conclusion Cadmium can dosedependently induced the oxidative damage in the mitochondria in rat liver, NAC has an antagonistic effect to cadmium.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第8期683-685,共3页
Journal of Environment and Health
