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一氧化氮调控剂ZX-5对血管内皮细胞eNOS和ERK蛋白表达的影响 被引量：1

Influence of ZX-5 as nitric oxide modulator on the expression of endothelial nitric oxide synthase and extracellular signal-regulated kinase protein of blood vessel endothelia cell
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摘要 目的:研究新型一氧化氮调控剂ZX-5作用后血管内皮细胞eNOS和ERK蛋白的表达,初步阐明ZX-5的作用机理。方法:用Western blotting测定ZX-5作用后血管内皮细胞eNOS和ERK蛋白的表达。结果:ZX-5能够增加eNOS的表达量,高剂量ZX-5可以增加ERK蛋白表达。结论:ZX-5可能通过ERK途径增加eNOS的表达(eNOS是ZX-5作用的靶点之一),具有开发成药物的潜力。 Objective To explore the influence of ZX-5 as NO modulator on the expression of nitric oxide synthase （eNOS） and extracellular signal-regulated kinase （ERK） protein of blood vessel endothelium cells. Method The expressions of eNOS and the ERK protein in blood vessel endothelium were determined by Western blotting. Result ZX-5 elevated the expression of eNOS, but had no distinguished effect on the ERK. Conclusion ZX-5 promotes the expression of eNOS via ERK. eNOS is one of the targets of ZX-5, and ZX-5 has a potentiality to be developed into medicine.
作者 王伟强 奚涛 沈子龙 林红英 吴国球
机构地区 中国药科大学 东南大学附属中大医院检验科
出处 《东南大学学报（医学版）》 CAS 2007年第6期417-420,共4页 Journal of Southeast University(Medical Science Edition)
关键词 一氧化氮调控剂 年龄相关性黄斑变性 一氧化氮合酶 胞外信号调节激酶蛋白 modulator of nitric oxide age-related macular degeneration nitric oxide synthase extracellular signalregulated kinase protein
分类号 R972.4 [医药卫生—药品] Q813.11 [生物学—生物工程]
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参考文献13

  • 1BYLSMA G,GUYMER R.Treatment of age-related macular degeneration[J].Optomytry,2005,88 (5):322-334.
  • 2张奕华,彭司勋.一氧化氮及其调控剂的研究[J].中国药科大学学报,2001,32(5):321-328. 被引量：14
  • 3XUAN B,XU X R,CHIOU G C,et al.Relationship between nitric oxide production and choroidal blood flow[J].J Ocular Pharmacol Therap,2003,19 (3):247-253.
  • 4JAFFE E A,NACHMAN R L,BECKER C G,et al.Culture of human endothelial cells derived from umbilical veins:identification by morphologic and immunologic criteria[J].J Clin Invest,1973,53 (12):2745-2756.
  • 5PARK J S,HONG G R,BAEK S W,et al.Expression and regulation of endothelial nitric oxide synthase by vascular endothelial growth factor in ECV 304 cells[J].Kor Med Sci,2002,17(2):161-167.
  • 6ROSSMANITH W G,HOFFMEISTER U,WOLFAHRT S,et al.Expression and functional analysis of endothelial nitric oxide synthase (eNOS) in human placenta[J].Molec Hum Reprod,1999,5 (5):487-494.
  • 7冯宁,陆国平,张曼玲,吴春芳,龚兰生,冯宗忱.西立伐他汀对血管内皮细胞一氧化氮合酶及细胞间黏附分子的作用[J].中华心血管病杂志,2002,30(5):308-311. 被引量：11
  • 8马歇克 D R,门永 J T,布格斯 R R,等.蛋白质纯化与鉴定实验指南[M].北京:科学出版社,2000:160.
  • 9CAMPOCHIARO P A,SOLOWAY P,RYAN J W,et al.The pathogenesis of choroidal neovascularization in patients with age-related macular degeneration[J].Molec Vision,1999,34(5):34-36.
  • 10TEZEL T H,BORA N S,KAPLAN H J.Pathogenesis of agerelated macular degeneration[J].Trends in Molecular Medicine,2004,10(9):417-420.

二级参考文献69

  • 1万梅,于占久.内皮衍生舒张因子对缺血（缺氧）、再灌注（复氧）心肌的保护作用[J].生理学报,1995,47(3):231-237. 被引量：20
  • 2[1]Gary L. Johnson, Razvan Lapadat. The protein kinase complement of the human genome. Science, 2002;296(6): 1911 ～1912.
  • 3[2]Walter Kolch. Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions. J.Biochem., 2000; 351: 289～ 305.
  • 4[3]Sun H., King A.J., Diaz, H.B., et al. Regulation of the protein kinase raf-1 by oncogenic ras through phosphatidylinositol 3- kinase, Cdc42/Rac and Pak. Curr. Bid.,2000; 10: 281～ 284.
  • 5[4]King A.J., Sun H., Diaz, B., et al. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338. Nature, 1998; 396:180～ 183.
  • 6[5]Maon C.S., Springer C.J., Cooper, R.G., et al. Serine and tyrosine phosphorylations cooperate in Raf-1, but not BRaf activation. EMBOJ., 1999; 18:2137～2148.
  • 7[6]卢建,余应年,徐仁宝.受体、信号转导系统与疾病.第1版.山东:科学技术出版社,2001:249～254.
  • 8[7]Scott T. Eblen, Jill K. Slack, Michael J., et al. Rac-PAK signaling stimulates exracellular signal-regulated kinase (ERK)activation by regulating formation of MEK1-ERK complexes.Molecular And Cellular Biology. 2002; 22(17): 6023～ 6033.
  • 9[8]Andrei V., Khokhlatchev, Bertram Canagarajah, et al.Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translation. Cell, 1998; 93: 605 ～ 615
  • 10[9]Mishima K, Inoue K, Hayashi Y. Overexpression of extracellular- signal regulated kinases on oral squamous cell carcinoma.Oral Oncol, 2002;38(5) :468～474.

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东南大学学报（医学版）
2007年 第6期

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