摘要
利用氮离子束和γ射线辐照处理粳稻日本晴和籼稻"9311",经过M1代损伤鉴定、M2代筛选和M3代重复鉴定,分别得到了740份和666份日本晴突变体,571份和781份"9311"突变体。获得的突变体发生了叶片、茎秆、穗部和籽粒、生理等性状的突变。在氮离子束辐照处理中,日本晴和"9311"分别在5.0×1016N+/cm2和2.5×1016N+/cm2剂量辐照时获得高突变率,为6.44%和6.38%;γ射线辐照处理中,2种材料均在150Gy剂量辐照时获得高突变率,分别为5.68%和9.44%。在本试验中,各高突变率群体均为辐照当代损伤最低的群体。新构建的突变体库将为水稻功能基因组学研究提供较好的基础材料。
The japonica rice cultivar ＂Nipponbare＂ and indica rice cultivar ＂9311＂ were treated by nitrogen ion beam implantation and y-rays irradiation to induce mutants for genetic and functional genomics researches. The damage effects were evaluated in M1 generation and M1 plants were harvested by single plant. Screening in M2 generation and identifying in M3 generation, 740 and 666 mutants in Nipponbare and 571 and 781 mutants in ＂9311＂, were induced by nitrogen ion beam and ＂γ-rays. Morphological variations at vegetative and reproductive stages, including leaf, stalk, panicle, seed and various physiological characters, were commonly observed in the two mutagenized populations. The mutation frequency of 6.44 % for Nipponbare at the ion beam dose of 5.0×10^16 N^＋/cm^2 and 6.38% for ＂9311＂ at 2.5 × 10^16 N^＋/cm^2 were observed, and for r-rays irradiation at dosage of 150Gy were 5.68% and 9.44% respectively. In the present study, the damages in M1 generation of each higher mutation population were least. So, this new mutant library, which was obtained by ion beam implantation and γ-rays irradiation, may be helpful for gene identification and gene functional analysis in future.
出处
《核农学报》
CAS
CSCD
北大核心
2008年第4期389-393,403,共6页
Journal of Nuclear Agricultural Sciences
基金
中国科学院方向性创新项目(KSCX-SW-324)
关键词
水稻
辐照
氮离子束
Γ射线
突变体库
rice
irradiation
N^＋ ion beam
y-rays
mutant library
