摘要
目的:探讨维药伊木萨克片对雄性大鼠阴茎勃起功能的影响及其机制。方法:性功能正常SD雄性大鼠用伊木萨克连续干预6周后,进行阿普吗啡(APO)阴茎勃起实验和交配实验,并检测阴茎海绵体窦压(ICP);用放射免疫法检测外周血清中睾酮(T)、促黄体生成素(LH)和促卵泡刺激素(FSH)含量,镜检睾丸的组织形态学改变;用免疫组织化学技术检测两组大鼠阴茎组织中eNOS、nNOS表达。结果:(1)与正常对照组相比较,伊木萨克组APO勃起所需时间显著缩短,插入次数显著增加,且基础ICP值与电刺激诱导ICP值均显著升高;(2)T水平在伊木萨克组显著高于正常对照组,而LH与FSH在两组间无显著性差异;(3)eNOS表达在伊木萨克组显著高于正常对照组,nNOS在两组间无显著性差异。结论:维药伊木萨克片能够显著提高正常大鼠的阴茎勃起功能,其机制可能和提高雄激素水平与eNOS表达有关。
Objective： To study the effect of the Uygur medicine Yi-Mu-Sa-Ke on the erectile function in male rats. Methods： M Sprague-Dawley rats with normal erectile function were administered with Yi-Mu-Sa-Ke for six weeks, after which the erection, mating and intracavernous pressure （ICP） were tested, testosterone （T）, luteinizing hormone （LH） and follicle-stimulating hormone （FSH） levels in their blood were determined, and the histo-morphological change in their testicles were examined and their eNOS and nNOS expressions were studied by means of SP irnmunohistochemistry. Results： （1） Compared with the control group, shorter APO-induced erectile response, more frequent mating activity and higher ICP and electrically induced ICP were recorded in Yi-Mu-Sa-Ke group. （2） The T level in the Yi-Mu-Sa-Ke rats was significantly higher, while LH and FSH levels showed no difference. （3） The eNOS expression in the Yi-Mu-Sa-Ke group was significantly higher than that in the control group, though there was no significant difference in the nNOS protein expression between the two groups. Con- elusion.. The fact that Yi Mu-Sa-Ke can significantly improve the erectile function in rats may be due to the drug to raise the level of androgen and increase eNOS protein expression.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2008年第4期579-582,共4页
Chinese Journal of Anatomy
基金
新疆科技厅重点实验室开放项目(XJDX0208-2006-04)
新疆高校科研计划科学研究重点项目(XJEDU2006I38)
