摘要
目的研究二甲基亚砜、丙酮、甲醇、异丙醇四种有机溶剂在斑马鱼模型抗血管生成药物筛选中作为助溶剂的适用性。方法以每种有机溶剂系列浓度处理发育12 h或24 h斑马鱼胚胎,到发育72 h观察胚胎血管生成、畸形、死亡状况,计数体节间血管,并计算畸形率和死亡率。结果有机溶剂中存活的斑马鱼胚胎体节间血管形态和数量正常,与对照一致。0.1%浓度二甲基亚砜、丙酮和1%浓度甲醇中斑马鱼胚胎无畸形和死亡状况,但随着浓度上升畸形率和死亡率均显著高于对照。0.1%浓度异丙醇中斑马鱼胚胎的畸形率和死亡率已显著高于对照。结论作为助溶剂,二甲基亚砜、丙酮适宜浓度为≤0.1%,甲醇适宜浓度为≤1%,异丙醇不适合用于斑马鱼模型抗血管生成药物筛选。
Objective To study the applicability of four kinds of organic solvents, dimethyl sulfoxide, acetone, methanol, isopropyl, as cosovlents in anti-tumor drug screening with zebrafish model. Methods zebrafish embryos at the stage of 12h or 24 h post-fertilization were treated with serial concentrations of each organic solvent. The state of angiogenesis, abnormality and death of the above zebrafish embryos were investigated by Counting the number of intersegmental vessels and calculating rates of abnormality and death until 72h post-fertilization. Results The shape and number of intersegmental vessels of living zebrafish embryos in each organic solvent are normal and identical with the control. Abnormality and death of zebrafish embryos are not observed in 0.1% concentrations of dimethyl sulfoxide, acetone and 1% concentrations of methanol, while rates of abnormality and death are significantly higher than the control with the increase of concentration. Abnormality and death rates of zebrafish embryos are significantly higher in 0.1% concentrations of isopropyl compared with the control. Conclusions Used as a cosolvent, the suitable concentration for dimethyl sulfoxide and acetone is below or equal to 0.1%, and for methanol is below or equal to 1%. Isopropyl is not fit for anti-angiogenesis drug screening with zebrafish model.
出处
《实验动物与比较医学》
CAS
2008年第4期238-242,共5页
Laboratory Animal and Comparative Medicine
基金
山东省科技攻关项目(2006GG3202038)
