摘要
信息素结合蛋白（pheromone binding proteins，PBPs）在昆虫信息素的识别、传递和处理过程中具有重要作用。本研究首次克隆r中华蜜蜂Apis cerana cerana的一个PBP基因Ac-ASP1（GenBank序列号为DQ449670），其预测蛋白具有典型的气味结合蛋白（OBPs）标志（即成熟肽含有6个保守的半胱氨酸）。利用real-timePCR技术对Ac-ASP1在中蜂不同组织和发育历期的时空表达谱进行了鉴定。绝对定量结果显示Ac-ASP1高丰度地表达于工蜂触角（2.07×10^6拷贝数/μg），而在其他组织（如头、胸、腹、翅及足）中呈低丰度表达（10^2拷贝数／μg）；相对定量结果显示Ac-ASP1在各发育历期如幼虫、蛹以及成虫发育早期（1-6日龄）均有大量表达，而在21日龄前后具有另外一个高丰度表达时期。这些结果可为明确Ac-ASP1在中蜂蜂王信息素信号识别传递过程中的作用提供参考。
Pheromone binding proteins （PBPs） play an important role in recognition, transmission and processing of insect pheromones. In this study, a cDNA named Ac-ASP1 encoding a PBP was cloned （from the Chinese native honeybee, Apis cerana cerana. The predicted protein has six conservative cysteines in mature peptide, a typical symbol of odorant binding proteins （OBPs）. The sequence had been submitted into GenBank and registered is the accession no. DQ449670. The profile of spatio-temporal expression of Ac-ASP1 was identified by real-time PCR. The results of absolute quantitation showed that Ac-ASP1 was expressed at a higher level in antenna （2.07 ×10^6 copy number/μg）, but at a less level in other tissues including head, thorax, abdomen, wings and legs （10^2 copy number/μg） ; while the results of relative quantitation revealed that Ac-ASP1 was expressed in two high abundance periods： from larva to the 6th day of adult and around the 21st day of adult. These results may provide the reference to ascertain the function of Ac-ASP1 involved in signal recognition and transmission of queen pheromone.
出处
《昆虫学报》
CAS
CSCD
北大核心
2008年第7期689-693,共5页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30270896)
浙江省自然科学基金项目(Y307597)
关键词
中华蜜蜂
工蜂
信息素结合蛋白
克隆
时空表达
实时定量PCR
Apis cerana cerana
worker bee
pheromone binding proteins （PBPs）
cloning
spatio-temporalexpression
real-time PCR
