摘要
目的建立多西紫杉醇脂质体包封率测定方法。方法多西紫杉醇的含量测定采用HPLC,色谱柱为Lichrospher-RPC18柱(150 mm×6.0 mm,5μm),流相为乙腈-水(60∶40),流速为1.0 mL.min-1,检测波长为231 nm,柱温为35℃。利用0.22μm微孔滤膜分离脂质体与游离药物,考察滤膜法测定脂质体包封率的可行性。结果该色谱条件下,多西紫杉醇得到良好分离,辅料不干扰药物的测定,各指标均符合测定要求。0.22μm微孔滤膜截留作用明显,能有效分离游离药物与载药脂质体。结论滤膜法简便、快捷,非常适合于多西紫杉醇脂质体的包封率测定。
OBJECTIVE To establish a method for determination of the entrapment efficiency of docetaxel liposomes, METHODS HPLC was established for the assay of docetaxel, Lichrospher-RP CI8 column （ 150 mm × 6, 0 mm,5 μm） was used, with acetonitrile-water （60： 40） as mobile phase, the flow rate was 1, 0 mL · min^-1, the detection wavelength was 231 nm, and the column temperature was 35 ℃, The 0.22 μm microspore film was used for the separation of docetaxel liposomes and free docetaxel, the feasibility of the method was evaluated, RESULTS Docetaxel could be separated well, the adjuvants did not interfere the determination of docetaxel, and all the concerning indexes according to the requests under such HPLC condition, The free docetaxel was obviously blocked by 0.22 μm microspore film; the method was effective for the separation of docetaxel liposome and free docetaxel. CONCLUSION The method is very suitable for determination of the entrapment efficiency of docetaxel liposomes.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第4期314-316,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
滤膜法
高效液相色谱法
多西紫杉醇
脂质体
包封率
filtration by microspore film
HPLC
docetaxel
liposomes
entrapment efficiency
