摘要
目的观察β-七叶皂苷钠对大鼠脑出血后脑水肿的影响。方法用立体定位法采用新鲜自体血注入大鼠尾状核建立脑出血模型,将实验大鼠随机分为治疗组和对照组;根据用药时间,两组各分成1、3、6、24 h组,治疗组于相应时间点经尾静脉注射七叶皂苷钠(2.5 mg/kg.d),对照组于相应时间点连续3天尾静脉注射生理盐水。干湿重法观察大鼠脑含水量以及血肿周围组织肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、基质金属蛋白酶-9(MMP-9)的表达。结果治疗组脑含水量明显低于对照组(P<0.05),各治疗组间相比差异无显著性(P>0.05)。对照组免疫荧光染色示TNF-α在出血灶周围组织广泛表达,而治疗组阳性细胞表达显著低于对照组(P<0.05),血肿周围组织IL-6表达与对照组相比差异无显著性(P>0.05),对照组MMP-9的表达显著高于治疗组(P<0.05)。结论β-七叶皂苷钠有抗脑出血后脑水肿的作用,其作用途径可能通过抗炎性损伤来实现。
Objective To observe the effect of β-aescinate administered on changes of edema after intracerebral hemorrhage in rats and to explore the mechanism.Methods Intracerebral hemorrhage rat models were established by stereotaxically injecting autologous blood at caudate nucleus.Rats were randomly divided into two groups： test group and control group.After operation,β-aescinate was injected by caudal vein in different time（1 h,3 h,6 h and 24 h） to test group（2.5 mg/kg·d） and sodium chloride was injected to control group in corresponding time.Brain water content was investigated and the expression of TNF-α,IL-6 and MMP-9 was measured in brain tissue.Results Water content in the perihematoma tissue in the test group was lower than that in the control group（P〈0.05）.However,there was no statistic significance between subgroups of treatment（P〉0.05）.Compared with the control group,the edema of neuron and stellate cell were lower in test group;the expression of TNF-α in brain tissue were obviously lower（P〈0.05） in test group.At the same time,the expression of IL-6 in brain tissue had no significant difference between the test group and the control group（P〉0.05）,but MMP-9 in the control group was higher than that in the test group（P〈0.05）.Conclusion β-aescinate has an evident protective effect on resisting edema after intracerebral hemorrhage in rats,which may be related to anti-inflammatory.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2008年第7期470-473,I0004,共5页
Journal of Beijing University of Traditional Chinese Medicine
关键词
七叶皂苷钠
脑出血
脑水肿
大鼠
aescinate
cerebral hemorrhage
brain edema
rats
