摘要
目的:建立灵敏可靠的高效液相色谱-荧光法进行丙泊酚血浆浓度的测定,考察丙泊酚静脉给药后母婴血浆浓度差异。方法:静脉注射丙泊酚注射液5min后,立即取母血、脐动脉血和脐静脉血,3500rpm离心10min,分离血浆。血浆样本用甲醇沉淀蛋白、高速离心后,取上清液采用高效液相色谱法进行分析,以甲醇-水(80:20,V/V)为流动相,流速1.0ml/min,荧光检测激发波长276nm,发射波长310nm。采用SPSS13.0软件对结果进行统计学分析。结果:丙泊酚血浆浓度在0.1～5.0μg/ml范围内线性关系良好(r=0.9975);低、中、高三个浓度日内变异RSD为4.49%～10.61%。脐静脉血、脐动脉血和母血中丙泊酚血浆浓度分别为(0.83±0.25)、(0.78±0.24)和(2.28±0.70)μg/ml。经统计学分析母血和脐血中丙泊酚血浆浓度差异显著(P<0.01),而脐动脉血和脐静脉血中丙泊酚血浆浓度的差异无统计学意义(P>0.05)。结论:本测定方法准确简便,灵敏可靠,适用于丙泊酚血浆浓度的分析测定。丙泊酚静脉滴注5min后,新生儿的血浆浓度明显低于母体血浆浓度。
Objective：To determine the concentration of propofol in plasma by HPLC with fluorescence detection, and then to study the difference between mother and infant.Methods：After administration of propofol injection, blood of mother and infant were collected and plasma were separated. The plasma was treatment with methanol and the supematant was analyzed by HPLC-fluorescence detector at 276/310 nm.The mobile phase consisted of methanol-water （80：20, WV）, at the flow of 1.0 ml/min.SPSS software was used to analyze the results. Results：The linear rang was 0.1-5.0 μg/ml and RSD of intra-day validation was 4.49%-10.61%. The plasma-concentrations of mother, umbilical vein and umbilical artery were （0.83±0.25）,（0.78±0.24）and （2.28±0.70） μg/ml, respectively. Plasma concentrations of mother and infant showed significant difference （P〈0.01）, but those of umbilical vein and umbilical artery had no statistical difference （P〉 0.05）.Conclusion：The method is simple, accurate and precise. It is suitable for the determination of propofol in human plasma. After administration,plasma concentrations of infant are significantly lower than those of mother.
出处
《中国医药导报》
CAS
2008年第25期13-16,共4页
China Medical Herald
