摘要
目的用长期脂肪乳剂灌胃的方法建立小鼠高脂血症动物模型,探讨长期脂肪乳剂灌胃可否造成小鼠高脂血症性骨质疏松,并探讨该类型骨质疏松骨生物力学的改变,探讨复方丹参合剂(DSC)对长期脂肪乳剂灌胃造成的小鼠高脂血症及骨质疏松是否有防治作用。方法SPF级2月龄KM小鼠50只,(雌雄各半,体质量(20±2)g,随机分成5组,每组10只。1组为普通饲料对照组,2组为脂肪乳剂组(脂肪乳剂10 ml/kg灌胃,共8周),3组为辛伐他汀组(脂肪乳剂+辛伐他汀组),4组复方丹参低剂量(DSC-L)组(脂肪乳剂+DSC-L组),5组复方丹参高剂量(DSC-H)组(脂肪乳剂+DSC-H组)。实验结束时测定血清TC、TG和HDL-c含量;左侧股骨用于骨羟脯氨酸、钙、磷的测定;右侧股骨用于骨生物力学测定。结果与普通饲料组相比脂肪乳剂组小鼠的TC、LDL-c和动脉粥样硬化指数(AI)均明显升高(P<0.01),HDL-c降低(P<0.01),TG无明显差异。脂肪乳剂组小鼠骨羟脯氨酸(Hyp)比值、骨Ca含量均明显下降(P<0.01),三点弯曲实验最大载荷、最大挠度和断裂载荷降低(P<0.05)。与脂肪乳剂组比较,DSC组小鼠AI降低(P<0.01),但还没达到正常组的水平,HDL-c明显升高(P<0.01),低、高剂量组间比较无统计学差异。DSC低、高剂量组骨Hyp比值、骨有机质比值均上升(P<0.05),与普通饲料组水平接近,但两治疗组间无差异;DSC高剂量组断裂载荷升高(P<0.05),但与普通饲料组仍有较大差异。结论脂肪乳剂长期灌胃可引起小鼠出现TC升高、HDL-c降低为特征的脂质代谢紊乱,并伴有高脂血症性骨质疏松症,复方丹参合剂可提高长期脂肪乳剂灌胃的小鼠血清HDL-c,对高脂血症所致骨质疏松有一定的防治作用。
Objective To observe the effects of Danshen root compound （DSC） on blood lipid and bone biomechanics in mice with hyperlipemia-induced osteoporosis. Methods Forty Kunming mice were randomized into 5 equal groups, and were given intragastric administration with distilled water （control）, lipid emulsion （LE） at the daily dose of 5 ml/kg, LE plus simvastatin, LE plus DSC at 5.0 g/kg （DSC-L group）, and LE plus DSC at 10.0 g/kg （DSC-H group）, respectively. Serum TC, TG, and HDL-c levels and left femur hydroxyproline, calcium and phosphate contents were measured in the rats, with the right femur taken for bone biomechanical test, Results Compared with those in the control group, serum TC, LDL-c and AI of the mice increased and HDL-c, Hyp and bone calcium decreased significantly （P〈0.0 1） with lowered bone biomechanical properties. Compared with those of the LE model group, Al decreased and HDL-c increased significantly in DSC-L and DSC-H groups （P〈0,01）, and the bone biomechanics in DSC-H group was improved. Conclusion Long-term intragastfic administration of lipid emulsion causes lipid metabolic disorder and induces osteoporosis due to hyperlipemia in mice. DSC can significantly increase HDL-c and partially prevent the occurrence of osteoporosis in mice.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第9期1550-1553,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30672470)
