摘要
目的:检测食管鳞状细胞癌(ESCC)组织中血管内皮生长因子-C(VEGF-C)及血小板生成因子-4(FLT-4)蛋白的表达。方法:应用免疫组织化学SP法检测50例ESCC组织及其相应的癌旁不典型增生组织(19例)及正常食管黏膜组织(50例)中VEGF-C与FLT-4蛋白的表达,并分析ESCC组织中2者的表达与患者临床病理因素的关系。结果:ESCC组织、癌旁不典型增生组织及正常食管黏膜组织中VEGF-C蛋白的阳性表达率依次降低,分别为82.0%(41/50)、47.4%(9/19)、26.0%(13/50),3组间比较,差异有统计学意义(P<0.05);FLT-4蛋白的阳性表达率依次降低,分别为72.0%(36/50)、36.8%(7/19)、18.0%(9/50),3组间比较,差异有统计学意义(P<0.05);VEGF-C蛋白的表达与肿瘤的浸润深度、淋巴结转移有关(P<0.05),FLT-4蛋白的表达与肿瘤的淋巴结转移有关(P<0.05)。ESCC组织中2者的阳性表达呈正相关(rs=0.536,P=0.000)。结论:VEGF-C和FLT-4与ES-CC的发生、浸润及转移有关,联合检测2者可望成为ESCC早期诊断和判断预后的分子指标之一。
Aim ： To explore the expression of VEGF-C and FLT-4 protein in esophageal squamous cell carcinoma（ ES- CC） tissue. Mathods：SP immunohistochemical method was used to detect the expression of VEGF-C and FLT-4 protein in 50 cases of ESCC tissue, 19 cases of adjacent atypical hyperplasia epithelium and 50 cases of normal esophageal epithelium. Results： The expression rates of VEGF-C protein decreased by turns in carcinoma, adjacent atypical hyperplasia epi- thelium and normal esophageal epithelium specimens which were 82.0% （41/50） ,47.4% （9/19） and 26.0% （13/50） respectively. There was significant difference among the 3 groups （ P 〈 0.05）. The expression rates of FLT-4 protein decreased, too, which were 72.0% （36/50） ,36.8% （ 7/19 ） , and 18.0% （9/50） respectively. There was significant differ-ence among the 3 groups（P 〈0.05）. The expression of VEGF-C protein was closely related with the infiltrative depth and lymphatic metastasis of ESCC（P 〈 0.05 ）, The expression of FLT-4 protein was closely related with the lymphatic metastasis of ESCC（P 〈 0.05）. The expression of VEGF-C protein was positively correlated with the expression of FLT-4 in ESCC tissue. Conclusion： VEGF-C and FLT-4 play important roles in the infiltration, metastasis and carcinogenesis of ESCC. Unitedexamining VEGF-C and FLT-4 can become one of molecular indexes in the early diagnosis and prognostic judgement of ESCC.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第5期865-867,共3页
Journal of Zhengzhou University(Medical Sciences)
