摘要
建立了染发剂中22种染料成分的高效液相色谱测定方法。使用Discovery？RP-Amide？C16柱（250mm×4.6mm，5μm），以乙腈-0.025mol/L磷酸盐缓冲液（pH6.0，含0.1%的庚烷磺酸钠离子对试剂）为流动相，流速为1.0mL/min；使用二极管阵列检测器（DAD），检测波长为260nm和280nm，柱温为25℃。该方法除了对低浓度的甲苯-2，5-二胺硫酸盐、2-甲基雷锁辛和N，N-二乙基甲苯-2，5-二胺HCl外，对其他各组分的含量测定值的相对标准偏差（RSD）均小于10%，加标回收率为77.6%~122.8%。该方法简便、准确、快速，适于氧化型染发剂中染料成分的检测。
A high performance liquid chromatographic（HPLC）method was developed for the determination of 22 components in hair dyes.The target analytes were separated on an amide bonded C16 silica column（250 mm×4.6 mm,5 μm）,employing acetonitrile-0.025 mol/L phosphate buffer（pH 6.0）containing 0.1% 1-heptanesulfonic acid sodium salt as the mobile phase and detected by a photodiode array detector（DAD）with the detection wavelengths of 260 nm and 280 nm.The flow rate was 1.0 mL/min and the column temperature was 25 ℃.The linear range was from 10 mg/L to 500 mg/L with good relationship.The relative standard deviations were less than 10%（except toluene-2,5-diamine sulfate,2-methylresorcinol,N,N-diethyltoluene-2,5-diamine HCl at low concentrations）and the recoveries were from 77.6% to 122.8%.The method is simple,rapid and accurate,and is suitable for the analysis of various hair dyes.
出处
《色谱》
CAS
CSCD
北大核心
2008年第5期554-558,共5页
Chinese Journal of Chromatography
基金
卫生部化妆品标准委员会研制项目
关键词
高效液相色谱法
染料
染发剂
high performance liquid chromatography（HPLC）
dyes
hair dyes
