摘要
目的探讨Fas／FasL途径在氟致人神经母细胞瘤SH—SY5Y细胞凋亡中的作用。方法用不同剂量氟化钠[NaF，0（对照）、20、40、80mg／L]对SH—SY5Y细胞进行染毒，24h后检测细胞存活率、凋亡率和Fas、FasL mRNA表达；选择40mg／L NaF组，观察在Fas受体激动剂（CH11）或拮抗剂（ZB4）作用下细胞凋亡率及Fas、FasL mRNA表达水平的改变。结果40、80mg／L组细胞存活率[（84．63±2．57）％、（69．04±5．63）％]明显低于对照组（100．00％），组间比较差异有统计学意义（P〈0．01）；细胞凋亡率随染毒剂量的升高呈上升趋势，40、80mg／L组细胞凋亡率[（8．54±1．95）％、（17．94±2．71）％]明显高于对照组[（3．32±1．33）％]，组间比较差异有统计学意义（P〈0．05）；NaF能不同程度地上调Fas、FasL mRNA表达，使Fas／β-actin[40mg／L组（0．94±0．51）、80mg／L组（0．99±0，12）]和Fast／β-actin[40mg／L组（0．96±0．42）、80mg／L组（0．99±0．24）]比值增加，与对照组[Fas／β-actin（0．50±0．33）、FasL／β-actin（0．58±0．23）]比较，差异均有统计学意义（P〈0．05）。在对细胞凋亡率和Fas、FasL mRNA表达水平的影响中，NaF与CH11之间存在协同作用（F值分别为32．89、18．46、14．69，P〈0．01），NaF与ZB4之间存在拮抗作用（F值分别为5．73、24．26、10．17，P〈0．05或〈0．01）。结论NaF可诱导SH—SY5Y细胞凋亡，Fas／FasL途径在NaF诱导SH—SY5Y细胞凋亡中起重要作用。
Objective To explore the effect of Fas/FasL pathway on fluoride-induced apoptosis in human neuroblastoma SH-SYSY cells. Methods The cell survival rate, percentage of apoptosis, and mRNA expression levels of Fas and FasL were measured respectively after the SH-SY5Y cells were exposed to 0 （control）,20,40,80 mg/L sodium fluoride （NaF） for 24 hours in vitro. Furthermore, the changes of the percentage of apoptosis and mRNA expression levels of Fas and FasL in 40 mg/L NaF-treated groups incubated with activating or neutralizing anti-Fas antibody（CHll or ZB4） also observed respectively. Results Compared with the control group（100.00%）, the cell survival rates in 40,80 mg/L NaF-treated groups[ （84.63 ± 2.57）%, （69.04 ± 5.63）%] were significantly lower（P 〈 0.01 ）. The percentage of apoptosis in 40,80 mg/L NaF-treated groups[（8.54 ± 1.95）%, （17.94 ± 2.71 ）%] were higher（P 〈 0.05） than that in the control group[ （3.32 ± 1.33）%], and increased with the dose of NaF. NaF could up-regulate Fas and FasL mRNA expression, and increased the Fas/β-actin [40 mg/L group （0.94 ± 0.51 ）, 80 mg/L group（0.99 ± 0.12）] and FasL/β-actin[40 mg/L group（0.96 ± 0.42）, 80 mg/L group（0.99 ± 0.24）] ratio, compared with the control [Fas/β-actin （0.50 ± 0.33）, FasL/β-actin（0.58 ± 0.23）], both the difference had statistical significances （P 〈 0.05）. NaF and CH11 had a synergistic effect on apoptosis and mRNA expression levels of Fas and FasL（F = 32.89,18.46,14.69, P 〈 0.01 ） while NaF and ZB4 had an antagonistic effect （F = 5.73,24.26, 10.17, P 〈 0.05 or 〈 0.01 ）. Conclusion NaF exposure can cause apoptosis in SH-SY5Y cells, and the Fas/FasL pathway may play an important role in NaF-induced apoptosis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第5期479-483,共5页
Chinese Jouranl of Endemiology
基金
国家自然科学基金（30671819）
新世纪优秀人才支持计划（NCET）资助（2005）
