摘要
[ Objective ] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum. [ Method] Three methods of Cutting Method, Liquid Nitrogen Method and Quartz Sand Method based on SDS method were employed to extract Artemisia abrotanum genomlc DNA from tender leaf at seedling stage, tender spike and old leaf at heading stage. The obtained DNAs were detected by absorbance detection, agarose gel and PCR amplification. [ Result ] Cutting Method performed better than the other two methods compared in purity, extracting cycle and cost, accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua.
传统的CTAB法成本高、毒性较大且操作繁琐，往往仅在需DNA量较大的RFLP等分析中采用。SDS提取的DNA产率接近CTAB产率的2倍，但纯度、完整性不如后者。常用的植物DNA简易提取方法有碱处理法及高温水煮法等。这些简易提取方法大都速度快、成本低，但提取DNA质量差、保存时间短、不适于PCR扩增。该文以SDS法为依据，在此基础上比较了3种方法的提取效果，从而摸索出一种快速有效的提取青蒿DNA的方法（以下简称“剪碎法”）。
基金
Project of Key Laboratory of Biological Resource Protection and Utilization in Hubei Province(2007025)
Open Fund of Hubei Key Laboratory of Biotechnology in Traditional Chinese Medicine in Hubei University(20060201)
Project of Hubei Institute for Nationalities~~
