摘要
目的:探讨骨髓基质细胞诱导神经干细胞移植至KA大鼠海马后对学习记忆障碍的改善作用,从而为干细胞移植治疗癫痫提供理论依据。方法:首先分离幼年大鼠骨髓基质细胞,并在特定的条件下培养、诱导分化为神经干细胞。然后用KA建立大鼠癫痫模型,之后把神经干细胞移植至KA致痫鼠的海马内,用Morris水迷宫测试大鼠的学习记忆能力。结果:癫痫移植组与癫痫未移植组相比幼年大鼠空间学习记忆能力有显著性差异(P<0.05)。结论:骨髓基质细胞诱导神经干细胞移植至KA幼年大鼠海马后对学习记忆障碍有明显的治疗作用,但其具体的作用机理有待进一步研究。
Objective： To study the recovery effect on study and memory dysfunction after bone marrow stromal cells inducing neural stem cells to transplant young rats＇hippecamp treated by Kanic acid and to approach the theory that stem cells transplantation cures epilepsy. Methods： At first the bone marrow stromal cells were segregated, cultured, induced and differentiated to neural stem cells in specific circumstance in rats. The rat＇ epilepsy model was established with kanic acid. At last the neural stem cells were transplanted to hippoeamp with epilepsy rats to test the study and memory ability of rats with Morris maze test. Results： The spatial study and memory was obvious difference in epilepsy transplantation group compared with those of controlled group （P 〈 0. 05）. Conclusion： The curing effect is obvious on study and memory dysfunction after bone marrow cells inducing neural stem cells to graft to hippocamp with epilepsy rats, but the mechanism is not clear till now.
出处
《中国妇幼保健》
CAS
北大核心
2008年第29期4188-4190,共3页
Maternal and Child Health Care of China
基金
北华大学科研基金(200733)
关键词
骨髓基质细胞
神经干细胞
颞叶癫痫
Bone marrow stromal cells
Neural stem cells
Temporal lobe epilepsy
