摘要
目的:建立牛胆汁中胆酸HPLC含量测定的方法,为制定中药炮制辅料牛胆汁的药用标准打下基础。方法:采用5C18-PAQ Waters柱(4.6mm×250mm,5μm),以甲醇-水-冰醋酸(72∶28∶0.2)为流动相,流速1ml/min;蒸发光检测器载气流速2.2L/min,漂移管温度85℃。结果:胆酸在1.648μg～4.944μg,呈良好线性关系(r=0.9998),平均回收率为99.48%,RSD为4.2%。结论:本方法准确,简便,重复性好,可用于牛胆汁中胆酸的含量测定。
Objective： to establish the assay method to detect the cholalic acid in bills bovina by HPLC to establish the base to institute drug standard of processing clruas adjuvant bills bovina. Methods： the 5C18 - PAQ Waters Collumn（4.6 mm×250 mm,5μm） was used and mobile phase was methanol-water-glacial acetic acid（72：28：0.2）. The flow rate was 1 ml/min. The evaporation photodetector flow rate of carrier gas was 2.2 L/min. The drift tube temperature was 85℃. Results： the cholalic acid had linear correlation during i. 648μg-- 4. 944 μg（ r = 0. 9998 ）. The average recovery was 99.48 % and the RSD was 4.2 %. Conclusion： this method is exact, convenient and good reproducibility and could use the assaying cholalic acid in bills hovina.
出处
《河南中医学院学报》
2008年第6期28-30,共3页
Journal of Henan University of Chinese Medicine
基金
科技部社会公益研究专项(编号:2005DIB1Z169)
关键词
牛胆汁
胆酸
蒸发光散射法
bilis bovina
cholalic acid
evaporation light scattering
