摘要
The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling（CK）,even the seedlings treated with lethal oxytetracycline concentration.
The effects of oxytetracycline on the morphology of the seedling with witches＇ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated. The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg· L^-1 oxytetracycline, and the pathogen could not be detected with PCR in the seedlings. The global DNA methylation level of diseased seedlings was lower than that of healthy one. The oxytetracycline treatment was able to increase the DNA methylation level, and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline. However the increased methylatlon of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling （ CK）, even the seedlings treated with lethal oxytetracycline concentration.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第9期152-156,共5页
Scientia Silvae Sinicae
基金
国家自然科学基金(30271082
30571496)
教育部高等学校博士学科点专项科研基金(20050466003)
河南省杰出青年基金(0612001900)资助项目
关键词
豫杂一号泡桐
丛枝病
幼苗形态
土霉素
DNA甲基化
Paulownia tomentosa × P. fortunei
witches＇ broom plantlet morphology
oxytetracycline
DNA methylation
