摘要
目的:以THP-1巨噬细胞源性泡沫细胞为研究对象,观察不同浓度黄芪多糖对胆固醇流出和ABCA1基因表达的影响。方法:将THP-1细胞诱导分化成泡沫细胞,用不同浓度黄芪多糖对其干预24h,γ计数仪检测胆固醇流出,RT-PCR及流式细胞仪检测ABCA1的表达。结果:黄芪多糖呈剂量依赖性(10-100mg/L)促进胆固醇流出;增加ABCA1的表达。结论:黄芪多糖可能通过增加ABCA1的表达而促进胆固醇流出。
AIM： To study the effect of astragalus polysaccharides （Aps） on cholesterol efflux in THP - 1 macrophage - derived foam cells. METHODS ： After exposed to Aps at different doses, cholesterol efflux and ABCA1 protein levels in cultured THP - 1 macrophage - derived foam cells were determined by a γ counter and flow cytometry, respectively. RESULTS ： Aps increased cholesterol efflux in THP - 1 macrophage - derived foam cells with dose dependent pattern and resulted in an increase in the expression of ABCA1 protein in THP - 1 macrophage - derived foam cells. CONCLUSION ： The increase in cholesterol efflux by Aps might be related to the up - regulation of ABCA1.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第10期2029-2032,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30670999)
关键词
黄芪多糖
巨噬细胞
胆固醇
ATP结合匣式转运子
Astragalus polysaccharide
Macrophages
Cholesterol
ATP binding cassette transporter
