摘要
Objective： To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern, the authors applied gene chip tech to filter variably expressed genes in hypothalamus. Methods： Rats were seperated into normal, model, acupuncture Heart Meridian group and acup Lung Meridian group randomly. Acute myocardial ischemia rat model was made with ligation left anterior descending branch of the coronary artery. After model succeed, select hypothalamus seperately and mixed the same group together of 3 rats. Then applied Rats U230A genechip refered by Affymetrix Co. to compare the variations between these groups. Results： To compare with normal group, differential expression genes and expression sequence tags （ESTs） in model group were 73 with signal log ratio ≥ 1 and 92 with signal log ratio ≤-1 , mainly included ion channel, calcium/ calmodulin-dependent protein kinase Ⅱ inhibitor, antigen and so on. Similarly, compared with model group, differential expression genes and expression sequence tags （ESTs） were 190 with signal log ratio ≥ 1 and 34 with signal log ratio ≤-1 in acupuncture Heart Meridian group, mainly included 5-hydroxytryptamine receptor, cellular metabolism, fatty, immuno reaction, G-protein coupled receptors, ion transport, signal transductions and so on, while in acupucnturc Lung Meridian, the number is 57 and 26 correspondly. Conclusion： There were exactly variations in hypothalamus mechanism that relate to acupuncture Heart and Lung Meridians.
目的:从基因水平揭示下丘脑中与针刺心经干预心肌缺血相关的基因表达谱,探索针刺心经抗干预心肌缺血的中枢作用机制。方法:采用结扎冠状动脉前降支法复制大鼠急性心肌梗死模型。比较心经组与模型组、模型组与正常组的心脏基因表达谱变化。结果:与正常组比较,模型组大鼠下丘脑差异表达2倍以上基因(包括 EST)中,上调表达73个,下调表达92个;与模型组比较,电针心经组下丘脑中2倍以上差异表达基因有190个上调,34个下调,涉及细胞代谢、脂质代谢、免疫反应、G 蛋白偶联受体、离子转运、信号转导等多种因子;而电针模型肺经组仅有57个基因表达上调,26个基因表达下调。结论:心经组、肺经组中大于2倍的差异表达基因的数目和类型有较大差异,提示针刺心经干预心肌缺血作用有相对特异性。
