摘要
目的观察组织胺对失血感染模型大鼠肠道细菌移位的影响。方法从十二指肠灌注大肠杆菌制成单纯感染模型,从颈静脉插管放血加十二指肠灌注大肠杆菌制成失血感染模型。大鼠随机分为假手术组、单纯感染组、失血感染组和1×10-6mol/L浓度的组织胺对以上各组的干预组共6组。组织胺干预组分别用1×10-6mol/L的组织胺对制成的模型大鼠进行肠内灌注。观察模型大鼠肠系膜淋巴结和肝脏匀浆体外培养后的细菌量,细菌移位率,小肠病理改变。结果假手术组有10%的组织培养为细菌阳性,单纯感染组有50%大鼠组织细菌阳性,失血感染组组织细菌培养的阳性率高达95%。组织胺各干预组则分别为5%、20%、25%,HA干预组与失血感染组相比均有显著性差异(P<0.01)。HA干预组肝及MLN平均组织含菌量与失血感染组相比均有统计学意义(P<0.05)。1×10-6mol/LHA干预组肠黏膜病理损伤较失血-感染组也明显减轻(P<0.01)。结论一定剂量的组织胺有保护肠黏膜屏障抑制细菌移位的作用。
Objective To observe the effects of Histamine on bacterial transloeafion（BT） and imestinal mueosal harrier in rats after hemorrhageinfection. Methods Sixty SD rats were divided randomly into 6 groups. 1 ×10^-6 mmol/L doses or saline were gayaged in rats subjected to hemorrhage combined with infection or controls. Bacterial cultures of mosentery lymph node （MLN） and liver were performed together with the measurement of portal endotoxin （ET） level in all animals. Furthermore, the injured degree of intestinal mueosa were classified according to the Chin＇s standard. Results The rate of bacterial transloeation was 10% in sham operated group, while it was 95% in henmrrlmge infection groupand. 5% ＆20% ＆.25% in sham operated ＋ HA groutxYcinfection ＋ HAgroup＆hemorrhage infection ＋ HA group respectively. Moreover, the average number of bacterial translocation to liver and MLN were 2.68 CFU/g,5.43 CEU/g in hemorrhage infection group, which is significantly higher compared with 1×10^-6 mmol/L HA （0.86 CFU/g, 1.58 CFU/g）, （ P 〈 O. 05）. Under light microscope, in comparsion with hemorrhageinfection group, intestinal mucosa damage in 1×10^-6 mmol/L HA became more slightly. （ P 〈 0.05）. Conclusion Appropriate dose of Histamine has the protective effect on intestinal barrier function and the inhibitory effect on bacterial translocation in hemorrhageinfection rats.
出处
《中国实验诊断学》
2008年第10期1223-1225,共3页
Chinese Journal of Laboratory Diagnosis
关键词
失血
感染
小肠黏膜
细菌移位
组织胺
hemorrhage
infection
intestinal mucosa
bacterial translocafion
histamine.
