摘要
本文分别对楸叶泡桐、兰考泡桐、鄂川泡桐和台湾泡桐的同品种、同方位和同高度的同一病株病叶、病健叶和健株叶片的蛋白质进行了ＳＤＳ聚丙烯酰胺凝胶电泳及其水解产物进行了分析研究。结果表明，４种感病泡桐植株的叶片内都出现了一条健康植株叶片内没有的、分子量为１２ＫＤ的蛋白质谱带，并且同一病株病叶内该蛋白质的量较病健叶的量多。此外，楸叶泡桐和台湾泡桐病株病叶、病健叶和健株叶片蛋白质的氨基酸组成也存在着明显差异。随叶片感病程度的加深，胱氨酸含量逐渐增多，而苯丙氨酸则逐渐减少。泡桐叶片内的这些变化可能与丛枝病的发生有一定联系。
This paper dealt with the SDS polyacrymide gel electrophoresis of proteins and analysis of the amino acids in the diseased and healthy leaves,at the same side and same height,of the same strain of Paulownia catalpifolia,P. elongata,P. albiphloea and P. kawakamii respectively. The results indicated that the leaves of 4 species of Paulownia tress with witches′ broom had one protein band,of which molecular weight was 12 KD,which did not appear in the healthy leaves free of phytoplasmas. Moreover,the protein quantity in the affected leaves was more than that in the healthy leaves free of phytoplasmas.Moreover,the protein quantity in the affected leaves was more than that in the healthy leaves free of phytoplasmas. At the same time,there was significant difference on the amino acids between the healthy and diseased leaves of P. catalpifolia and P. kawakamii. The amount of cystine in the affected leaves was higher than that in the healthy leaves,but the change of amount of phenylalanine in the affected and healthy leaves was contrary. These changes of proteins and amino acids in the leaves might be related to the witches′broom of the Paulownia trees.
出处
《林业科学研究》
CSCD
北大核心
1997年第6期570-573,共4页
Forest Research
基金
河南省杰出青年基金
关键词
泡桐
丛枝病
蛋白质
氨基酸
防治
Paulownia witches′ broom protein amino acid
