摘要
目的探讨食管下端括约肌（LES）肌间内神经及肌层的发育规律与小儿胃食管反流发生的可能关系。方法分别取孕18d（E18）、孕20d（E20）胎鼠，出生后1d（P1）、7d（P7）、14d（P14）、21d（P21）、30d（P30）、成年鼠（A）的LES及食管体（EB），廊用常规HE染色法及蛋白基冈产物9．5（PGP9．5）免疫组化法，结合图像分析技术，定性、定量研究LES肌间内神经与肌层的发育变化。结果随日龄的增加，LES和EB的肌间神经元、神经丛密度均逐渐下降，神经元胞体增大、胞浆着色加深，在哺乳期前后神经元成熟度达成年水平。在各个日龄组LES肌间内神经元密度和神经丛密度均较EB稠密。LES肌层出生后发育迅速，成年后各肌层继续增厚，环肌层发育总是优于纵肌层。结论LES肌间内神经及肌层发育不一致，神经发育以哺乳期变化最为显著，至生后30d基本发育成熟。
Objective To explore the developmental regularity of Auerbach＇s plexus neurons and muscular layers in lower esophageal sphincter （LES） and its possible relationship with children＇s gastroesophageal reflux （GER）. Methods Collecting LES and esophageal body（EB） from rats in various development stages： embryonic age 18th day（E18）, 20th day（E20）, postnatal 1st day（P1）, 7th day （P7）, 14th day（P14）, 21st day（P21）, 30th day and adult（A） ; the routine HE staining method and Protein Gene Product 9. 5 （PGP9. 5） immunohistochemistry with imaging analysis were applied. And a qualitative and quantitative description of the Auerbach＇s plexusneurons and muscular layers development in LES was obtained. Results With the age increasing, neurons and neural plexus neurons of LES and EB decreased, pericaryon turned bigger, cytoplasm was deeper stained; the maturity of neurons reached adult level after lactation. Compared with EB, neuron and neural plexus were more intensive in LES. The muscular layers of LES had blastoprolepsis after birth, and both muscular layers keep on developing after the adult stage with better development of circular layer than that of longitudinal muscle layer. Conclusions In lactation, Auerbach＇s plexusneurons of IrES develop remarkably till 30th day after birth. Development of muscular layers and Auerbach＇s plexusneurons are not at equal pace.
出处
《中华小儿外科杂志》
CSCD
北大核心
2008年第10期624-627,共4页
Chinese Journal of Pediatric Surgery
关键词
食管括约肌
下段
肠神经系统
肌肉发育
Esophageal sphincter, lower
Enteric nervous system
Muscles development
