摘要
目的研究在水提取过程中酸枣仁粉碎前后其所含有效成分酸枣仁皂苷A的溶出以及安神功效是否有差异。方法采用HPLC法,Agilent C18色谱柱(250mm×4.6mm,5μm),流动相:甲醇-水(65∶35),流速1.0mL/min;蒸发光散射检测器;漂移管温度110℃;载气流速2.4L/min。采用协同睡眠、自主活动等指标,观察粉碎的酸枣仁与未粉碎的酸枣仁对小鼠的作用。结果未经粉碎的酸枣仁在其水提样品中未检测出酸枣仁皂苷A,粉碎后的酸枣仁在其水提样品中的酸枣仁皂苷A含量为0.0090%;粉碎后的酸枣仁对戊巴比妥钠有较好的协同睡眠作用。结论临床应用酸枣仁宜粉碎。
Objective To study the dissolving difference of jujubaside A and tranquilizing effect crushed Semen Ziziphi Spinosae in water extraction. Methods Crushed Semen Ziziphi Spinosae and Semen Ziziphi Spinosae were decocted and the content of jujubaside A in different samples was inspected by HPLC. The experiment was carried out on Agilent C18 column （250 mm× 4.6mm, 5 μm）, using methanol-water （65 ： 35） as mobile phase at a flow rate of 1.0 mL/min. The detector was ELSD. The temperature of drift tube for ELSD was 110 ℃ and the flux of N2 was 2.4 L/min. The mAce given two different samples were observed of assisting sleeping and voluntary action. Results Jujubaside A was not found in sample of Semen Ziziphi Spinosae, and the content of jujubaside A in sample of crushed Semen Ziziphi Spinosae was 0.009 0%. The sample of crushed Semen Ziziphi Spinosae could assist sleeping well. Conclusion Semen Ziziphi Spinosae should be crushed for clinical use.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第11期39-41,共3页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
酸枣仁
酸枣仁皂苷A
安神作用
Semen Ziziphi Spinosae
jujubaside A
tranquilization
