摘要
建立紫外分光光度法测定γ-聚谷氨酸含量的方法。利用γ-聚谷氨酸水解成谷氨酸，在pH9．6硼砂缓冲溶液中，能与四氯对苯醌形成1：1的络合物，该络合物在波长350nm处有最大吸收，通过测定其吸光度确定γ-聚谷氨酸含量。结果表明，γ-聚谷氨酸在8～12μg／mL范围内线性关系良好（r为0．9981），平均回收率为99．5％，相对标准偏差为1．0％。该方法简便准确、重现性好，可作为γ-聚谷氨酸含量的测定方法。
This paper establishes an ultraviolet spectrophotometric method for determination of γ-polyglutamic acid. After hydrolysis γ- polyglutamic acid became aminoglutaminic acid which could shape comoles compound （1 ： 1） in pH 9.6 of borax buffer solution with chloranil. The comoles compound had absorption maximum at 350 nm. Thus we determined γ-polyglutamic acid by utilizing its absorbance. There was good linearity in the range of 8-12 μg/mL of γ- PGA （r=0. 998 1） and the average recovery was 99.5% with RSD of 1.0 %. The method is simple, reliable and reproducible which can be used to assay γ - PGA.
出处
《青岛大学学报（工程技术版）》
CAS
2008年第3期86-89,共4页
Journal of Qingdao University(Engineering & Technology Edition)
关键词
Γ-聚谷氨酸
络合反应
紫外分光光度法
四氯对苯醌
γ- polyglutamic acid
complexation reaction
ultraviolet spectrophotometry
chloranil
