摘要
获得纯化的人类和动物表皮干细胞不仅可为构建有生理功能的人工皮肤提供种子细胞,对了解皮肤组织器官发育、肿瘤疾病发生也具有重要意义,是转基因和组织工程技术中的重要靶细胞。利用慢周期性和自我更新能力是鉴别表皮干细胞最基本且较可靠的实验手段,但这两种方法的应用很不方便。目前位于表皮干细胞表面的糖蛋白已作为其特异性标志而受到关注。因表皮干细胞的特异性分子标志、识别标准、分布定位及体外培养条件下生物学特性的变化等诸多方面都还存在争议,限制了其基础研究与临床应用。
Acquiring pure human and animal epidermal stem cells can not only provide seed cells for constructing artificial skin with physiologic function, but also have a significant meaning for comprehending skin tissue organ development and occurrence of neoplasms diseases. Pure human and animal epidermal stem cells are an important target cells in the technology of transgenes and tissue engineering. Utilizing slow periodicity and the ability to self-renewal is more fundamental and reliable experiment methods, which can discriminate epidermal stem cells, but the application of these two methods is not convenient. At present, glycoprotein, which locates the surface of epidermal stem cells, has already been a key focus as specific landmark. The variation of epidermal stem cells in many ways already have disputes, such as the specific molecular landmark of epidermal stem cells, the standardization of recognition, distribution and location, bionomics in vivo condition and so on, which limits the fundamental research and clinical application.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第43期8567-8570,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科技资源平台建设项目(2001DEA1006)~~
