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咖啡短体线虫人工繁殖方法研究 被引量:2

Study on methoddogy of monoxenically reproduction of Pratylenchus coffea
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摘要 利用愈伤组织法对咖啡短体线虫进行了人工繁殖,并分析讨论了温度、培养时间、接种量对繁殖量的影响。试验结果表明,以25℃为最适温度,20℃与30℃线虫繁殖量不理想;以10条接种量为最适接种量,其次为5条接种量;繁殖量与所设计接种时间成正比。 Pratylenchus coffea was monoxenically reproduced on carrot disk culture,and the effect of time, temperature, and inoculum density on reproduction were discussed in the study.
出处 《广东农业科学》 CAS CSCD 2008年第11期58-60,共3页 Guangdong Agricultural Sciences
关键词 咖啡短体线虫 愈伤组织法 人工繁殖 Pratylenchus coffea carrot disk culture monoxenically reproduction
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