摘要
目的:探讨芹菜素对局灶性脑缺血再灌注损伤后核转录因子-κB(NF-κB)、诱导型一氧化氮合酶(iNOS)动态表达的影响。方法:将大鼠135只随机分为假手术组(S组)、模型组(M组)及芹菜素组(A组),后2组按再灌注时间不同又分为再灌注24h、48h、72h和7天各4小组,总共9小组,每组15只大鼠。采用线栓法制备大鼠左侧大脑中动脉缺血(1.5h)再灌注模型。光镜电镜观察脑组织病理形态改变,免疫组织化学法检测NF-κBp65蛋白表达,比色法检测iNOS活性。结果:大鼠脑缺血再灌注后M组各时间点NF-κBp65表达增多(P<0.01)、iNOS活性增高(P<0.01)。芹菜素在脑缺血再灌注后72h和7天有下调NF-κBp65的表达(P<0.05),降低iNOS活性的作用(P<0.05)。M组脑缺血再灌注后各个时间点皮层和海马细胞肿胀、细胞间质水肿,有空泡形成,尤以缺血再灌注后48h最为明显,而神经细胞均有核固缩、碎裂、溶解,以缺血再灌注后24h为著。芹菜素有减轻脑缺血再灌注后组织细胞水肿的作用,对神经细胞超微结构改变不明显。结论:芹菜素的神经保护作用可能与抑制大鼠缺血再灌注后脑组织中NF-κB的表达及iNOS活性有关。
Objective：To study the neuroprotective effect of apigenin on transient focal cerebral ischemia and repeffusion injury in rats ,which was characterized by its influence on nuclear factor-κappaB （NF -κB ） and inducible nitric oxide synthase （iNOS） expression. Methods：One hundred and thirty five male Spragur - Dawley rats were randomized into 9 groups ： sham - operated group （ S, n = 15 ） ; model groups （ group M24h , group M48h, group M72h, group M7d, n = 15 ） and apigenin- treated groups（ group A24h, group A48h ,group A72h, grouop A7d, n = 15 ）. The transient focal cerebral ischemia and reperfusion（1.5hours） model in rat was established with insertion of thread embolish into middle cerebral artery. The cellular and subcellular morphology under optic microscope and electromicroseope, the expression of NF -κB 1365 by immuno - histoehemistry （ ICH ） and the activity of inducible nitric oxide synthase （iNOS） by colorimetric method were measured. Results ：The expression of NF -κB p65 were significantly higher in group M （ P 〈 0.01 ） , the positive expressions of NF-κB p65 in group A72h and A7d were obviously lower than ones in group M （ P 〈 0.05 ）. The activities of iNOS were significantly higher in group M than ones in group S respectively（ P 〈 0.01 ）, the activities of iNOS in group A72h and group A7d were significantly lower than ones at the corresponding time points in group M respectively （ P 〈 0.05 ）. Group M showed obvious intracellular and intercellular edema and vacuolization in ischemic cortexes and hippocampuses, especially in group M48h. There were karyopyenosis and organelle broaden and dissolution and vacuolization in glias and neurons, especially in group M24h. The cellular edema in group A was lessened compared to one in group M, cellular morphologlc change was scarcely observed between group A and group M. Conclusion：Apigenin may play a neuroprotective effect which was related with depression of NF -κB activation and of the iNOS generation in transient focal cerebral isehemia and reperfusion of rats.
出处
《中华中医药学刊》
CAS
2008年第11期2438-2441,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省教育厅资助项目(200220448)
温州市科技局资助项目(S2002A079)
