摘要
目的对浙江省临床分离猪链球菌血清2型（SS2）菌株进行89K候选致病岛检测。方法用多种特异性引物进行PCR扩增检测，同时对89K的3个扩增片段进行测序，并结合有关资料进行生物信息学和流行病学分析。结果8株SS2菌株，均检出89K（1＆2）、89K（3＆4）、89K（5＆6）候选致病岛特异片段和种特异性16S rDNA、cps2J、mrp毒力基因，和1998年江苏省暴发疫情的菌株结果一致。ZJ0501号SS2分离株89K候选致病岛的3个PCR扩增片段序列和1998年江苏省暴发疫情的菌株有99％以上的相似性，其中编码DNA重组蛋白的序列片段和病乳链球菌及无乳链球菌有较高的同源性。结论近年来在浙江省临床分离SS2菌株中，存在89K候选致病岛片段。
Objective To identify the presence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis serotype 2 （SS2） strains isolated from patient in Zhejiang province. Methods Genes and DNA fragments were amplified by PCR, using specific primers, and three amplified fragments of the 89K sequence were directly sequenced. The results were analyzed using software related to bioinformatics and epidemiology. Results 8 strains of SS2 all contained 89K sequence, cps2J and mrp virulent genes,and species-specific 16S rDNA. 3 amplified fragments of 89K candidate pathogenicity island of SS2 ZJ0501 were above 99 % similar to SS2 strain identified from outbreaks in Jiangsu in 1998, and the gene fragment of coding DNA recombinant protein in the 89K sequence was highly homological with that of S. dysgalactiae and S. agalactiae. Conclusion In recent years SS2 strains isolated from patients with clinical symptoms in Zheiiang province had been detected to have contained candidate pathogenicy 89K DNA fragment.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2008年第11期1110-1113,共4页
Chinese Journal of Epidemiology
关键词
猪链球菌血清2型
聚合酶链反应
89K片段
Streptococcus suis serotype 2
Polymerase chain reaction
89K DNA fragment
