摘要
目的观察食管癌患者机体免疫状态的变化。方法采用流式细胞技术检测65例哈族患者T淋巴细胞亚群（Th1、Th2、CD3^＋、CD4^＋、CD8^＋细胞），采用双抗体夹心酶联免疫吸附试验（ELISA）法检测血清中白细胞介素（IL）-2、干扰素（IFN）-γ、IL4、IL10、sIL-2R和肿瘤坏死因子（TNF）-β水平。结果食管癌患者外周血Th1／Th2细胞比值降低（P〈0．05），其中Th1细胞表达的IL-2、IFN-γ等细胞因子水平低于正常对照组；Th2细胞表达的IL4、IL10细胞因子水平高于正常对照组（P〈0．05）。T细胞介导的细胞免疫及血清sIL-2R和TNF—β水平存在改变和紊乱，其程度与肿瘤TNM分期相关，其特征为：CD3^＋、CD4^＋细胞减少（P〈0．05），而CD8^＋细胞增加（P〈0．01）；CD4^＋／CD8^＋细胞比值下降（P〈0．01），血清sIL-2R和TNF—β水平升高（P〈0．05）。食管癌根治术后1个月CD3^＋、CD4^＋细胞回升，CD8^＋细胞回降，同时血清sIL-2R和TNF-β水平降低（P〈0．01）。结论食管癌患者外周血Th1／Th2细胞比值降低，细胞因子表达失衡，患者存在免疫功能抑制。
Objective To observe the changes in immune state in Kazakh patients with esophageal squamous cell cancer （ESCC）. Methods Flow cytometry was used to examine the subgroups of T lymphocytes. ELISA assay was used to determine the serum levels of IL-2, INF-γ, IL-4, IL10, sIL-2R and TNF- β. Results Th1/Th2 ratio of peripheral blood was significantly lower in esophageal cancer group （ P 〈 0.05）. IL-2 and IFN-γ expressed by Thl were significantly lower than in control group, while IL-4 and IL- 10 expressed by Th2 were significantly higher （ P 〈 0.05 ）. T cell-mediated cellular immunity was disordered, and the degree was related to TNM stage. The characteristics were as follows ： CD3 ^＋ and CD4^＋ were decreased and CD8^＋ increased （ P 〈 0. 05 ） ; the Th1/Th2 ratio was significantly decreased （ P 〈 0.05 ） , sIL-2R and TNF-β were increased （P 〈 0.05）. One month postoperation, CD3^＋ and CD4^＋ were increased, CD8 ^＋ decreased, and sIL-2R and TNF-β decreased （ P 〈 0.01 ）. Conclusion Dynamic observation and surveillance of ratio of CD4^＋/CD8^＋ and serum levels of IL-2, INF-γ, IL-4,IL-10, sIL-2R and TNF-β is a sensitive index to judge the immune function ,TNM stage, prognosis, post-operational effectiveness and early detection of recurrence of Kazakh patients with EC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第11期1463-1465,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30260049）
关键词
食管癌
T淋巴细胞
免疫功能
Esophageal carcinoma
T cell
Immunofunction
