摘要
在磷酸盐缓冲溶液中研究了邻苯二胺在玻碳电极表面的聚合过程,探讨了各氧化还原峰的变化机理,用扫描电镜表征了聚邻苯二胺膜的形态结构。发现制备的聚邻苯二胺膜修饰电极对H2O2有显著的电催化还原特性,线性回归方程为：Δipa（μA）=-1.63＋1.07cH2O2（mmol.L^-1）（R=0.9947,n=19）,线性范围为：5.89×10^-2mmol.L^-1～44.4 mmol.L^-1,检出限为：0.02 mmol.L^-1（3S/k）,这对与产生H2O2的氧化酶相结合制备出响应各种底物的电化学生物传感器非常重要。考察了制备条件对膜电极电催化还原活性的影响,发现只有在弱酸条件下制备的膜电极才有较高的电催化活性。探讨了影响膜电极电催化还原灵敏度的因素,并考察了膜电极的稳定性。
The polymerization process of o-phenylenediamine at a glassy carbon electrode surface in phosphate buffer solution was studied, the changes of every redox peak were discussed and the poly-o-phenylenediamine membrane morphology was characterized using scanning electron microscopy. It＇ s discovered that the poly-o-phenylenediamine modified electrode had significant electrocatalytic activity toward the reduction of H2O2. The linear regression equation is Alp, （ μA） = 1.63 ＋ 1.07cH2O2 （ mmol· L^-1 ） （R =0. 9947,n = 19） and the linear range for the determination of H2O2 is 5.89× 10^-2mmol· L^-1 -44.4 mmol · L^-1 with a detection limit of 0. 02 mmol·L^-1 （3S/k） ,which is very important for the preparation of sensitive electrochemical biosensor with a response toward various substrates by combination with various oxidases producing hydrogen peroxide. Various preparing conditions affecting the electrocatalytie activity of the membrane electrode were investigated and found that only in weak acidic conditions the membrane electrode had high electro-catalytic activity. The factors influencing the membrane electrode elctrocatalytie activity were discussed and the stability of membrane electrode was inspected.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2008年第11期1430-1435,共6页
Chemical Research and Application
基金
广东省自然科学基金博士科研启动基金项目(5300842)资助
华南农业大学校长科学基金项目(2005K119)资助
关键词
聚邻苯二胺
玻碳电极
电催化
过氧化氢
poly （ortho-phenylenediamine）
glassy carbon eletrode
eleetrocatalysis
hydrogen
