摘要
目的:建立复方利血平片中维生素B_1、维生素B_6、泛酸钙和氢氯噻嗪含量测定的HPLC法。方法:采用phenomenex的Synergi Hydro-RP色谱柱(150 mm×4.6 mm,4 μm),以乙腈、0.02 mol·L^(-1)磷酸氢二钾溶液和0.005 mol·L^(-1)庚烷磺酸钠(用磷酸调节pH至3.0±0.05)为流动相,采用梯度洗脱,流速为1.0 mL·min^(-1),用二极管阵列检测器检测,检测波长210nn,柱温36℃。结果:维生素B_1的线性范围为7.752～77.52 μg mL^(-1)(r=0.9992),精密度RSD为0.99%(n=7),重现性RSD为1.62%(n=7),平均回收率为98.63%,RSD为1.59%(n=9);维生素B_6的线性范围为7.580～75.80μg·mL^(-1)(r=0.9995),精密度RSD为0.62%(n=7),重现性RSD为1.17%(n=7),平均回收率为99.17%,RSD为1.26%(n=9);泛酸钙的线性范围为7.904～79.04 μg·mL^(-1)(r=0.9997),精密度RSD为0.58%(n=7),重现性RSD为0.95%(n=7),平均回收率为98.37%,RSD为0.92%(n=9);氢氯噻嗪的线性范围为25.62～256.2 μg·mL^(-1)(r=0.9995),精密度RSD为0.19%(n=7),重现性RSD为1.36%(n=7),平均回收率为99.12%,RSD为1.06%(n=9)。结论:本方法操作简便,结果准确、可靠,可同时测定复方利血平片中维生素B_1、维生素B_6、泛酸钙和氢氯噻嗪的含量。
Objecttive：To establish the method for the determination of VitaminB1, VitaminB6, Pantothenic acid and Hydrochlorothiazide in Compound Reserpine Tablets. Method：The separation was performed on phenomenex Synergi Hydro -RP column（4.6 mm × 150 mm,5 μm）using gradient elution with acetonitrile 0. 02 mol·L^-1potassium dihydrogen phosphate and 0. 005 mol·L^-1 heptanesulfonic acid sodium salt as mobile phase;the flow rate was 1.0 mL·min^-1 The detection was set at 210 nm and the column temperature was maintained at 36 ℃. Resuits：ALL the results for the standards curve, the average recovery and the separation showed htat the method is satisfactory. Conclusion：The method is simple, fast and accurate for analysis of Compound Reserpine Tablets .
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第11期1906-1908,共3页
Chinese Journal of Pharmaceutical Analysis
