摘要
目的：采用HPLC测定食品中咖啡因的含量。方法：根据样品类型采用不同的处理方法，以Symmetry Shield RPC18柱为固定相，甲醇／水为流动相，检测波长为273nm。结果：咖啡因与其它杂质均能达到基线分离，其色谱峰形好。咖啡因的浓度在0．006—0．12mg／ml时与峰面积成良好的线性关系（Y=-1．39×10^5＋3．40×10^7X，r=0．9995），平均回收率为94．5％～105％，方法精密度RSD为2．30％、2．60％和2．57％（n=6）。结论：所用方法简便、准确、重复性好，适用于食品中咖啡因的测定。
Objective： To study the determination method of caffeine in some kinds of food. Methods ： The content of caffeine in different kinds of food was determined by HPLC with Symmetry Shield RPC18 as stationary phase, Methanol/H2O as mobile phase and detective wavelength at 273 nm. Results： Caffeine and other peaks were separated well and the peak shape was presented well. There was a good linearity within the range of 0. 006 to 0. 12 mg/ml of caffeine. The average recovery was 94.5% - 105%. The RSD was 2. 30% ,2. 60% and 2.57% （n = 6）. Conclusion：This method is simple, accurate and reproducibile. It is suitable for the determination of caffeine in food.
出处
《中国卫生检验杂志》
CAS
2008年第11期2194-2196,共3页
Chinese Journal of Health Laboratory Technology
