摘要
目的:探讨L-肉碱(LC)在奥硝唑(ORN)所致雄性大鼠生殖系统氧化损伤中的保护作用。方法:40只雄性SD大鼠随机均分为5组,分别连续灌胃20d。A组(对照组):0.5%羧甲基纤维素钠(溶剂);B组:400mg/kgORN;C组:800mg/kgORN;D组:ORN(400mg/kg)+LC(100mg/kg);E组:ORN(800mg/kg)+LC(100mg/kg)。末次给药24h后,麻醉处死所有大鼠,分别检测各组睾丸、附睾组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)水平及总抗氧化能力(TAC)。结果:B组、C组及E组大鼠附睾MDA水平明显高于A组(P均<0.01),睾丸中MDA水平则各组间没有差异;B组、C组及E组睾丸、附睾SOD水平和TAC则分别显著低于A组(P均<0.05),而D组与A组相比无显著性差异;与C组相比,E组大鼠附睾MDA水平显著降低(P<0.01),而TAC则显著升高(P<0.05);虽然E组大鼠附睾、睾丸SOD水平及睾丸中的TAC与C组比无统计学差异,但有升高的趋势。结论:LC可保护大鼠生殖系统对抗ORN所致的氧化损伤,对大鼠生殖功能具有一定的保护作用。
Objective： To explore the protection of L-carnitine on the reproductive function of male rates which dealed with ornidazole （ORN）. Metheds： Forty male SD rats were randomly divided into five groups, 8 in each. Group A（the control）： 0.5% carboxymethylcellulose solution; group B： 400 mg/kg ornidazole （ORN）; group C： 800 mg/kg ORN; group D： ORN 400 mg/kg＋LC100 mg/kg; group E： ORN 800 mg/kg ＋ LC100 mg/kg. All rats were treated via gastric gavage for 20 d consecutively. After this treatment, the rats were killed and the concentrations of malondialdehyde （MDA）, superoxide dismutase （SOD） and total antioxidant capacity （TAC） were tested in the testis and epididymis tissues. Results： In epididymis, compared with group A, there was singnificant increase （P〈0.01） in groups B, C and E about MDA level, but there was no difference in testis about MDA level among these groups; SOD level and TAC in group A were hingher than those in groups B, C and E, respectively （P〈0.05）, but compared with group D, there was no difference in epididymis and testis about MDA, SOD levels and TAC （P〉0.05）; and compared with group C, MDA level in epididymis in group E was singnificant decreased （P〈0.01）, while that of TAC was distinctly increased （P〈0.05）, although there was no difference in group E in epididymis SOD level and testis TAC, it was increased obviously. Conclusion： LC can protect reproductive system of SD rats to opposition oxidative damage that ornidazole-induced.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2008年第11期656-659,共4页
Reproduction and Contraception
