摘要
背景他汀类药物具有多种临床功效包括调脂、改善内皮功能等,有报道表明他汀类药物可以防治压力负荷增大引起的心肌肥厚,但其确切机制尚不明确。目的观察阿托伐他汀对压力负荷导致的心肌肥厚的保护作用,并探讨其可能的机制。方法缩窄腹主动脉的方法制备大鼠心肌肥厚模型。Wistar 大鼠随机分为未治疗组(n=10)、阿托伐他汀组(n=10)、假手术组(n=10)、空白对照组(n=10)。阿托伐他汀组术后每日灌胃给予阿托伐他汀2 mg/kg,余各组给予生理盐水灌胃。实验结束后处死大鼠,测定其心脏/体质量、左心室/体质量及病理切片左室壁厚度和心肌细胞直径。通过 RT-PCR 方法检测心肌组织中心肌营养素1(CT-1)和白细胞介素18(IL-18)的表达。结果未治疗组[(174±9)mmHg]收缩压明显高于对照组[(112±15)mmHg](P<0.01),虽然阿托伐他汀组[(169±8)mmHg]血压仅有不明显下降(P>0.05),但能减轻心脏质量/体质量[阿托伐他汀:(3.2±0.2)×10^(-3)比末治疗组(4.2±0.29)×10^(-3),P<0.05]、左心室质量/体质量[阿托伐他汀:(2.2±0.09)×10^(-3)比未治疗组(2.8±0.27)×10^(-3),P<0.05]、阿托伐他汀还降低心肌中炎症因子,如 CT-1[阿托伐他汀:(0.244±0.042)比未治疗组(0.371±0.054),P<0.05]、IL-18 mRNA 含量[阿托伐他汀:(0.231±0.071)比未治疗组(0.462±0.038),P<0.05]及心肌细胞直径[阿托伐他汀:(9.74±0.38)μm比未治疗组(10.94±0.65)μm,P<0.05]。结论阿托伐他汀对压力负荷导致的心肌肥厚有明显的保护作用,同时伴有炎症因子水平的降低,说明阿托伐他汀的保护作用可能与其抗炎作用有关。
Background A multitude of studies reported statins prevent the myocardial hypertrophy induced by pressure overload, hut the mechanism is unclear. Objective To study the protective effect of atorvastatin on the myocardial hypertrophy caused by pressure overload and it＇s anti-inflammation effect. Methods Forty Wistar rats were randomized to untreated group（n= 10）, atorvastatin group（2 mg/kg·d, by gavage, n= 10）, sham operation group （n= 10） and control group（n= 10）. Ventricular hypertrophy model was achieved by surgical constricting the abdominal aorta. All rats were sacrificed 8 weeks after the operation and the heart weight to body ratio, left ventricular weight to body ratio, and the size of cardiomyocyte were determined. The expressions＇of interleukin 18（IL-18） and cardiotrophin.1 （CT-1） in the cadiocyte were assessed by reverse transcription polymerase chain reaction（RT-PCR）. Results Compared with control group, SBP in myocardial hypertrophy group was significantly increased（ 174 ± 9 vs control 112 ± 15 mmHg, P 〈 0.01 ）, whereas atorvastatin group did not decreased SBP（169±8 mmHg）. However, atorvastatin decreased the heart weight to body ratio[atorvastatin： （3.2±0.2））〈 10^-3 vs untreated group： （4.2±0.3））〈 10^-3 , P〈0.05], the left ventricular weight to body Patorvastatin： （2.2±0. 1） ）〈 10^-3 vs untreated group： （2.8±0. 3） ）〈 10^-3, P〈0.05], and concomitantly decreased the expressions of CT-1 （atorvastatin： 0. 244±0. 042 vs untreated group ： 0.371 ±0. 054, P〈0.05） and IL-18 （atorvastatin ： 0.23 ] ; 0.071 vs untreated group ： 0. 462±0. 038, P〈0.05）, and the diameters of cadiocytes （atorvastatin： 9.74±0.38μm vs untreated group：10.94±0.65μm, P〈0.05）. Conclusion Atorvastatin can prevent the myocardial hypertrophy caused by pressure overload which was associated with its anti-inflammation effect.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2008年第11期1028-1032,共5页
Chinese Journal of Hypertension
关键词
阿托伐他汀
心肌肥厚
保护作用
炎症
Atorvastatin
Ventricular hypertrophy
Protective effect
Inflammation
