摘要
研究采用乙酰丙酮分光光度法测定水产品中的甲醛含量,对样品的前处理过程进行了比较系统的探讨,确定了比色测定参数,并对其用于快速测定水产品中甲醛含量的可行性进行了讨论。实验结果表明,用冰浴浸泡法前处理样品时,最佳浸泡时间为10 min,样液pH值用100 g·L-1氢氧化钾溶液调至6;沸水浴中最佳显色时间为3 min,比色波长为412 nm;当甲醛含量在0～2.5μg.mL-1范围内时,该方法具有很好的线性关系(R2=0.9998);与蒸馏法前处理样品相比,该方法的加标回收率比较高,在89.50%～94.25%之间,而前者为64.17%～76.92%;该方法的RSD(%)为2.93%,样品的最低检出浓度为0.9 mg·kg-1。
Formaldehyde in aquatic products was determined by acetyle acetone speetrophotometry in the present research. The eolorimetric parameters and the preprocessing of samples in the method were studied, and the feasibility of using acetyle acetone speetrophotometry as a method in rapid determination of formaldehyde in aquatic products was discussed as well. Results showed that the optimal soaking time was 10 min during ice incubation, then soaking solution pH value was adjusted to 6 with 100 g·L^-1 KOH solution, and the optimal colorimetric time was 3 min at a wave length of 412 nm. The linear relation between absorbency and formaldehyde dosage was very good （R^2 = 0. 9998 ） while the formaldehyde content varied within a range of 0- 2. 5 μg·mL^-1. The recovery of the present method was 89. 50% -94. 25% , while the recovery of the distillation method was only 64. 17% -76. 92%. Comparing with the latter method, the recovery of present method was much better. RSD （%） of the present method was 2. 93% , and the minimum detectability of formaldehyde in samples was 0. 9 mg·kg^-1.
出处
《南方水产》
2008年第6期26-32,共7页
South China Fisheries Science
基金
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2008TS042007TS11)
关键词
甲醛
乙酰丙酮
分光光度法
水产品
formaldehyde
aeetyle acetone
spectrophotometry
aquatic product
